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[使用抗p30血清鉴定人类精液污渍]

[Identification of human semen stains using anti-p30 serum].

作者信息

Hou Y P, Wu M Y

出版信息

Hua Xi Yi Ke Da Xue Xue Bao. 1989 Jun;20(2):144-6.

PMID:2480326
Abstract

The present communication describes the identification of human semen stains using immunodiffusion test with anti-p30 serum. In order to evaluate objectively the value of the immunodiffusion test for the identification. Random sampling and blind study were used. The results indicated that the immunodiffusion test was more accurate than sperm detection. It was proved that p30 was a useful human semen marker especially in semen stains of azoospermia and vaseligation. The vaginal secretion and saliva in mixed stains did not interfere with the result of the immunodiffusion. The human semen stains can be differentiated from the animal semen stains with anti-p30 serum. Therefore, it is concluded that the demonstration of p30 in a suspected stain is the best method for human semen identification up to now.

摘要

本报告描述了使用抗p30血清免疫扩散试验鉴定人类精液污渍的方法。为了客观评估免疫扩散试验的鉴定价值,采用了随机抽样和盲法研究。结果表明,免疫扩散试验比精子检测更准确。事实证明,p30是一种有用的人类精液标志物,尤其在无精子症和输精管结扎后的精液污渍中。混合污渍中的阴道分泌物和唾液不干扰免疫扩散结果。用抗p30血清可将人类精液污渍与动物精液污渍区分开来。因此,得出结论:在可疑污渍中检测到p30是目前鉴定人类精液的最佳方法。

相似文献

1
[Identification of human semen stains using anti-p30 serum].[使用抗p30血清鉴定人类精液污渍]
Hua Xi Yi Ke Da Xue Xue Bao. 1989 Jun;20(2):144-6.
2
[Identification of sperm stains by a specific seminal plasma protein].[通过一种特定的精浆蛋白鉴定精液污渍]
Sud Med Ekspert. 1988 Apr-Jun;31(2):25-6.
3
The detection of protein p30 in seminal stains by means of thin-layer immunoassay.运用薄层免疫分析法检测精液污渍中的蛋白质p30。
J Forensic Sci. 1984 Oct;29(4):1173-7.
4
[Preparation and identification of anti-p30 serum].[抗p30血清的制备与鉴定]
Hua Xi Yi Ke Da Xue Xue Bao. 1988 Jun;19(2):112-5.
5
[The measurement of p30 level in the normal human seminal plasma by the Beckman immunochemistry system using anti-30 serum].[使用抗 - 30 血清通过贝克曼免疫化学系统测定正常人精浆中的 p30 水平]
Hua Xi Yi Ke Da Xue Xue Bao. 1990 Sep;21(3):274-6.
6
Postcoital detection of a male-specific semen protein. Application to the investigation of rape.性交后男性特异性精液蛋白的检测。在强奸案调查中的应用。
N Engl J Med. 1985 Feb 7;312(6):338-43. doi: 10.1056/NEJM198502073120603.
7
An evaluation of gamma-glutamyl transpeptidase (GGT) and p30 determinations for the identification of semen on postcoital vaginal swabs.对γ-谷氨酰转肽酶(GGT)和p30检测用于鉴定性交后阴道拭子上精液的评估。
J Forensic Sci. 1985 Jul;30(3):604-14.
8
[Detection of ABO blood group of seminal stains mixed with vaginal secretion or blood by a method using NCF coated with anti alpha 2-SGP serum--forensic immunological studies of body fluids and secretions, report XXXVI].[采用抗α2-SGP血清包被的硝酸纤维素膜法检测混有阴道分泌物或血液的精液斑的ABO血型——体液和分泌物的法医免疫学研究,报告XXXVI]
Nihon Hoigaku Zasshi. 1988 Dec;42(6):504-8.
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[The detection test for spot of seminal fluid using anti-gamma-seminoprotein serum and anti-beta-microseminoprotein serum--forensic immunological studies of body fluids and secretion, report XXXI].[使用抗γ-精蛋白血清和抗β-微精蛋白血清检测精液斑——体液与分泌液的法医免疫学研究,报告XXXI]
Nihon Hoigaku Zasshi. 1987 Oct;41(5):426-33.
10
[Demonstration of seminal stains by enzyme immunoassay using peroxidase-conjugated anti-gamma-seminoprotein (gamma-Sm) Fab'--forensic immunological studies of body fluids and secretion, report XXIV].[使用过氧化物酶偶联抗γ-精蛋白(γ-Sm)Fab'通过酶免疫测定法检测精液斑——体液和分泌物的法医免疫学研究,报告二十四]
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