Masibay A S, Lappas N T
J Forensic Sci. 1984 Oct;29(4):1173-7.
The detection of p30 by means of an indirect thin-layer immunoassay (TIA) is described. Extracts from 20 samples can be analyzed in approximately 2 h with a detection limit of approximately 50 ng. The p30 protein was detected in seminal stains which had been stored at room temperature for six months and at 130 degrees C for 4 h. Blood, saliva, urine, perspiration, and tears did not interfere with the method. The reliability of the method was demonstrated in a blind study.
本文描述了通过间接薄层免疫测定法（TIA）检测p30的方法。20个样本的提取物大约可在2小时内进行分析，检测限约为50纳克。在室温下储存6个月以及在130摄氏度下储存4小时的精液污渍中均检测到了p30蛋白。血液、唾液、尿液、汗液和眼泪均不干扰该方法。在一项盲法研究中证明了该方法的可靠性。
