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Improved O-serotyping method for Serratia marcescens.

作者信息

Gaston M A, Pitt T L

机构信息

Central Public Health Laboratory, London, England.

出版信息

J Clin Microbiol. 1989 Dec;27(12):2702-5. doi: 10.1128/jcm.27.12.2702-2705.1989.

DOI:10.1128/jcm.27.12.2702-2705.1989
PMID:2480362
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC267112/
Abstract

In a previous study, we found that some O serotypes of Serratia marcescens, as defined by agglutination tests, were not based on lipopolysaccharide (LPS) O antigens. We developed a dot enzyme immunoassay with a high degree of LPS specificity and tested 104 distinct clinical strains. Only 7 of the 24 existing O antigens were found in more than one strain: O12/O14 (30.8% of strains examined), O21 (12.5%), O8 (8.7%), O6/O7 (5.8%), O4 (3.8%), O18 (2.9%), and O9 (2.9%). Two new antigens, S1254 (13.5%) and S3255 (3.8%), were also found. Agglutination tests with O antisera identified the LPS antigen in only 36 strains. Prodigiosin production was restricted to serotypes O8, O6, and S3255 and strains with a rough or semirough LPS phenotype. Dot immunoassay appears to offer greater accuracy than agglutination tests for serotype identification in S. marcescens.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0f6/267112/2c265637a42d/jcm00072-0095-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0f6/267112/43e829c42b00/jcm00072-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0f6/267112/2c265637a42d/jcm00072-0095-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0f6/267112/43e829c42b00/jcm00072-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0f6/267112/2c265637a42d/jcm00072-0095-b.jpg

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本文引用的文献

1
Relationship between pigment producibility and drug resistance in Serratia marcescens.粘质沙雷氏菌色素产生能力与耐药性之间的关系。
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Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels.银染聚丙烯酰胺凝胶中沙门氏菌脂多糖化学型之间的形态异质性。
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A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels.
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Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
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Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity.分型系统鉴别能力的数值指标：辛普森多样性指数的一种应用
J Clin Microbiol. 1988 Nov;26(11):2465-6. doi: 10.1128/jcm.26.11.2465-2466.1988.
6
O-antigen specificities of the serotype strains of Serratia marcescens.粘质沙雷氏菌血清型菌株的O抗原特异性
J Clin Microbiol. 1989 Dec;27(12):2697-701. doi: 10.1128/jcm.27.12.2697-2701.1989.
7
Compatible results obtained from biotyping and serotyping in Serratia marcescens.从粘质沙雷氏菌的生物分型和血清分型获得的相符结果。
J Clin Microbiol. 1979 Oct;10(4):425-32. doi: 10.1128/jcm.10.4.425-432.1979.