Aucken H M, Pitt T L
Laboratory of Hospital Infection, Central Public Health Laboratory, London, England, United Kingdom.
J Clin Microbiol. 1993 May;31(5):1286-9. doi: 10.1128/jcm.31.5.1286-1289.1993.
We have applied the technique of lipopolysaccharide (LPS) profiling in sodium dodecyl sulfate-polyacrylamide gel electrophoresis to the typing of 124 isolates of 12 gram-negative species from suspected outbreaks of infection. LPS was prepared by proteinase K digestion or micro-phenol-water extraction. A total of 11 of the 12 species gave clear ladder band profiles, the exception being Acinetobacter baumannii. When compared with conventional typing for Enterobacter cloacae, Pseudomonas aeruginosa, and Serratia marcescens, LPS profile type alone was sufficient to allow relatedness or distinguishability of isolates to be established, and this was corroborated by serotype and phage type data. Serologically nontypeable isolates invariably lacked O repeating units and thus could not be classified by their silver stain profile. We conclude that LPS profiling is useful for the epidemiological investigation of small clusters of isolates in order to determine whether or not cross-infection between patients has occurred.
我们已将十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中的脂多糖（LPS）分析技术应用于对12种革兰氏阴性菌的124株分离株进行分型，这些分离株来自疑似感染暴发。LPS通过蛋白酶K消化或微量酚-水提取制备。12个菌种中的11个呈现出清晰的阶梯状条带图谱，鲍曼不动杆菌是个例外。与阴沟肠杆菌、铜绿假单胞菌和粘质沙雷氏菌的传统分型方法相比，仅LPS图谱类型就足以确定分离株之间的相关性或可区分性，血清型和噬菌体分型数据也证实了这一点。血清学上无法分型的分离株总是缺乏O重复单元，因此无法通过其银染图谱进行分类。我们得出结论，LPS分析对于小群体分离株的流行病学调查很有用，以便确定患者之间是否发生了交叉感染。
