Rustad O J, Kaye V, Cerio R, Zachary C B
Dermatology Department, University of Minnesota, Minneapolis 55455.
J Dermatol Surg Oncol. 1989 Dec;15(12):1262-7. doi: 10.1111/j.1524-4725.1989.tb03145.x.
Precise identification and localization of tumor is the key to success in Mohs micrographic surgery. In a pilot study, we demonstrated improvement in tumor definition and staining characteristics after formalin fixation of cryostat sections (postfixation) when compared with unfixed specimens. We further investigated the benefits of postfixation with a series of tissue fixatives and with varying fixation times. In all cases, postfixation was found to be beneficial. No improvement was noted by extending the postfixation time beyond 1 minute.
肿瘤的精确识别和定位是莫氏显微外科手术成功的关键。在一项初步研究中，我们发现与未固定的标本相比，低温恒温器切片经福尔马林固定（后固定）后，肿瘤的清晰度和染色特征有所改善。我们进一步研究了使用一系列组织固定剂和不同固定时间进行后固定的益处。在所有情况下，均发现后固定是有益的。将后固定时间延长至1分钟以上未观察到进一步改善。
