Humphreys T R, Nemeth A, McCrevey S, Baer S C, Goldberg L H
Department of Dermatology, Baylor College of Medicine, Houston, Texas, USA.
Dermatol Surg. 1996 Aug;22(8):693-7. doi: 10.1111/j.1524-4725.1996.tb00619.x.
Hematoxylin and Eosin (H&E) is the most common stain used for Mohs frozen sections. Toluidine blue (T-blue) is a metachromatic stain that has been frequently utilized for this purpose.
To compare the cytologic and stromal staining patterns of each stain for Mohs frozen sections of squamous cell carcinoma (SCC) and basal cell carcinoma (BCC), and to identify the advantages and disadvantages of each technique.
T-blue revealed stromal change associated with the presence of BCC and SCC. H&E provided more prominent visibility of individual cell keratinization and necrosis, which are common features seen in SCC.
We found T-blue to be fast and effective in identifying mucopolysaccharides in stroma associated with basal cell carcinoma. For this reason, T-blue is our preferred stain for BCCs, while H&E can provide greater ease of identification of histologic features of SCCs.
苏木精和伊红（H&E）是莫氏冷冻切片最常用的染色剂。甲苯胺蓝（T-蓝）是一种常用于此目的的异染性染色剂。
比较鳞状细胞癌（SCC）和基底细胞癌（BCC）莫氏冷冻切片中每种染色剂的细胞和基质染色模式，并确定每种技术的优缺点。
T-蓝显示出与BCC和SCC存在相关的基质变化。H&E能更清晰地显示单个细胞的角化和坏死，这是SCC常见的特征。
我们发现T-蓝在识别与基底细胞癌相关的基质中的黏多糖方面快速有效。因此，T-蓝是我们用于BCC的首选染色剂，而H&E能更便于识别SCC的组织学特征。
