Biomedicine Programme, School of Health Sciences, Universiti Sains Malaysia Kubang Kerian 16150, Malaysia.
Department of Chemical Pathology, School of Medical Sciences, Universiti Sains Malaysia Kubang Kerian 16150, Malaysia.
Food Sci Nutr. 2013 Jan;1(1):83-9. doi: 10.1002/fsn3.10. Epub 2013 Jan 8.
In this article, the novel inventive steps for the extraction and quantification of sulfated glycosaminoglycan (GAG) from Acanthaster planci starfish, generally known as crown-of-thorns (COT), are reported. Starfish have been implicated with collagenous distributions within their body anatomy, thus making it a prima facie fact searching for the possibility that GAGs can be isolated from COT. In this study, total-, N-, and O-sulfated GAGs were extracted from three anatomical regions of the COT (integument, internal tissue, and coelomic fluid) and comparison was made. The result showed that body region of COT seemed to contain higher amount of sulfated GAGs as opposed to the arm region (55.79 ± 0.65 μg/mg was the highest amount in the body extracted from its coelomic fluid and 32.28 ± 3.14 μg/mg was the highest amount in the arm extracted from its internal tissue). COT's integument and coelomic fluid from its body region possessed the highest total of sulfated GAGs content with no significant difference (P < 0.05) between the two. All GAGs from COT comprised a higher percentage of N-sulfated GAGs than its counterpart, the O-sulfated GAGs. When compared with a similar previous study that used sea cucumbers as the sulfated GAGs source, COT possessed more total sulfated GAGs content per milligram as compared with the sea cucumber generally. This result seems to unveil this marine species' advantage per se pertaining to GAGs extraction biomass applicability. Thus, COT could now be the better alternative source for production technology of total-, N-, and O-sulfated GAGs.
本文报道了从棘冠海星（通常称为棘冠海星）中提取和定量硫酸化糖胺聚糖（GAG）的新发明步骤。海星的身体解剖结构中存在胶原分布，因此可以初步推断 GAG 可以从棘冠海星中分离出来。在这项研究中，从棘冠海星的三个解剖部位（表皮、内部组织和体腔液）中提取了总 GAG、N-硫酸化 GAG 和 O-硫酸化 GAG，并进行了比较。结果表明，棘冠海星的身体部位似乎比腕部部位含有更高量的硫酸化 GAG（从体腔液中提取的身体部位的最高含量为 55.79±0.65μg/mg，从内部组织中提取的腕部部位的最高含量为 32.28±3.14μg/mg）。棘冠海星的表皮和体腔液从身体部位提取的总硫酸化 GAG 含量最高，两者之间没有显著差异（P<0.05）。棘冠海星的所有 GAG 均包含较高比例的 N-硫酸化 GAG，而 O-硫酸化 GAG 的比例较低。与使用海参作为硫酸化 GAG 来源的类似先前研究相比，棘冠海星每毫克总硫酸化 GAG 含量更高。这一结果似乎揭示了这种海洋物种在 GAG 提取生物量应用方面的优势。因此，棘冠海星可能是总 GAG、N-硫酸化 GAG 和 O-硫酸化 GAG 生产技术的更好替代来源。
