Koide M, Okabayashi Y, Hasegawa H, Tani S, Fujisawa T, Nakamura T, Fujii M, Otsuki M
Nihon Shokakibyo Gakkai Zasshi. 1989 Oct;86(10):2419-24.
We developed a specific and sensitive bioassay for measuring plasma cholecystokinin (CCK) in human and investigated CCK response after a test meal in patients with chronic pancreatitis. Treatment with cycloheximide increased the sensitivity and responsiveness of isolated rat pancreatic acini to CCK-octapeptide (CCK-8) and thus plasma levels of CCK-8 as low was 0.17 pM were detectable. Fasting plasma CCK levels in normal subjects as CCK-8 equivalents were 0.75 +/- 0.25 pM and rose to a peak of 6.2 +/- 0.68 pM at 45 min after a test meal consisting of 400 ml milk and 2 boiled eggs. Basal and stimulated plasma levels of CCK-8 in patients with non-calcified chronic pancreatitis were significantly higher than those in normal subjects. In contrast, postprandial responses of plasma CCK-8 in patients with calcified chronic pancreatitis was significantly low compared to those in control subjects, although basal plasma levels were not significantly different from those in controls.
我们开发了一种用于测量人体血浆胆囊收缩素（CCK）的特异性和敏感性生物测定法，并研究了慢性胰腺炎患者在试餐后的CCK反应。用放线菌酮处理可提高分离的大鼠胰腺腺泡对CCK八肽（CCK-8）的敏感性和反应性，因此可检测到低至0.17 pM的血浆CCK-8水平。正常受试者空腹血浆CCK水平以CCK-8当量计为0.75±0.25 pM，在由400毫升牛奶和2个煮鸡蛋组成的试餐后45分钟升至6.2±0.68 pM的峰值。非钙化性慢性胰腺炎患者的基础和刺激后血浆CCK-8水平显著高于正常受试者。相比之下，钙化性慢性胰腺炎患者餐后血浆CCK-8的反应与对照组相比显著降低，尽管基础血浆水平与对照组无显著差异。
