Wurm Alexander, Nogler Michael, Ammann Christoph G, Coraça-Huber Débora C
Experimental Orthopaedics, Medical University of Innsbruck, Salurnerstrasse 15, 6020, Innsbruck, Austria.
Int Orthop. 2014 Jul;38(7):1513-7. doi: 10.1007/s00264-014-2349-3. Epub 2014 May 8.
The aim of this study was to quantify the amount of bone morphogenic protein 7 (BMP-7) in bone samples in different storage and treatment conditions used in bone banks and thereby evaluate the benefit of this test as a routine measure before bone grafting.
Fresh as well as frozen bone chips, each with and without antibiotic impregnation, were screened for their BMP-7 content. Human bone chips were produced from femoral heads of two female donors who had undergone total hip replacement surgery. The amount of BMP-7 was detected using a commercially available enzyme-linked immunosorbent assay (ELISA) test.
There were no significant differences between groups in samples obtained from the first femoral head. Bone-chip samples derived from the second femoral head showed significant differences between groups. The actual amount of these differences was small and most likely biologically irrelevant. It is important to note that there was a significant difference between groups when comparing both femoral heads, reflecting donor-to-donor variability.
ELISA testing for BMP-7 as a qualitative measurement of bone grafts should be considered a routine quality-control test for bone banks.
本研究的目的是量化骨库中不同储存和处理条件下骨样本中骨形态发生蛋白7（BMP - 7）的含量，从而评估该检测作为骨移植前常规措施的益处。
对新鲜和冷冻的骨碎片进行筛选，每种骨碎片又分为含抗生素和不含抗生素的，检测其BMP - 7含量。人骨碎片取自两名接受全髋关节置换手术的女性供体的股骨头。使用市售的酶联免疫吸附测定（ELISA）检测法检测BMP - 7的含量。
从第一个股骨头获取的样本组间无显著差异。来自第二个股骨头的骨碎片样本组间存在显著差异。这些差异的实际量很小，很可能在生物学上不相关。需要注意的是，比较两个股骨头时组间存在显著差异，这反映了供体间的变异性。
将BMP - 7的ELISA检测作为骨移植的定性测量方法，应被视为骨库的常规质量控制检测。
