快速酶联免疫吸附试验检测不同小型哺乳动物中的汉坦病毒特异性抗体。
Rapid enzyme-linked immunosorbent assay for the detection of hantavirus-specific antibodies in divergent small mammals.
机构信息
Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaíso 2373223, Chile.
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523, USA.
出版信息
Viruses. 2014 May 6;6(5):2028-37. doi: 10.3390/v6052028.
We assessed the utility of an enzyme-linked immunosorbent assay (ELISA) for the detection of hantavirus-specific antibodies from sera of Oligoryzomys longicaudatus, the principal reservoir of Andes virus (ANDV), using an antigen previously developed for detection of antibodies to Sin Nombre virus (SNV) in sera from Peromyscus maniculatus. The assay uses a protein A/G horseradish peroxidase conjugate and can be performed in as little as 1.5 hours. Serum samples from Oligoryzomys longicaudatus collected in central-south Chile were used and the assay identified several that were antibody positive. This assay can be used for the rapid detection of antibodies to divergent hantaviruses from geographically and phylogenetically distant rodent species.
我们评估了酶联免疫吸附测定（ELISA）检测长爪沙鼠（Oligoryzomys longicaudatus）血清中汉坦病毒特异性抗体的效用，该鼠种是安第斯病毒（Andes virus，ANDV）的主要宿主，使用的抗原是先前为检测 Peromyscus maniculatus 血清中针对辛诺伯病毒（Sin Nombre virus，SNV）的抗体而开发的。该检测法使用蛋白 A/G 辣根过氧化物酶缀合物，可在短短 1.5 小时内完成。我们使用了在智利中南部采集的长爪沙鼠血清样本，该检测法鉴定出了一些抗体阳性的样本。该检测法可用于快速检测来自地理和系统发育上不同的啮齿动物物种的不同汉坦病毒的抗体。
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