Valeriano V D, Parungao-Balolong M M, Kang D-K
Department of Animal Resources Science, Dankook University, Cheonan, Korea.
J Appl Microbiol. 2014 Aug;117(2):485-97. doi: 10.1111/jam.12539. Epub 2014 Jun 2.
In this report, we characterized the probiotic potential of Lactobacillus mucosae LM1, focusing on its in vitro mucin-adhesion abilities.
Screening assays were used to evaluate LM1. Previous studies on Lact. mucosae species have been performed, but few have examined the ability of this species to adhere to and colonize the intestinal mucosa. Thus, adhesion, aggregation and pathogen inhibition assays of LM1 along with microbial adhesion to solvents (MATS) assay were carried out in comparison with another putative probiotic, Lactobacillus johnsonii PF01, and the commercial strain, Lactobacillus rhamnosus GG. Based on MATS assay, the cell surfaces of the lactobacilli strains were found to be hydrophobic and highly electron-donating, but the average hydropathy (GRAVY) index of predicted surface-exposed proteins in the LM1 genome indicated that most were hydrophilic. LM1 showed the highest adhesion, aggregation and hydrophobicity among the strains tested and significantly inhibited the adhesion of Escherichia coli K88 and Salmonella enterica serovar Typhimurium KCCM 40253. Correlations among adhesion, aggregation and hydrophobicity, as well as between coaggregation and displacement of E. coli, were observed.
Increased adhesion may not always correlate with increased pathogen inhibition due to various strain-specific mechanisms. Nevertheless, LM1 has promising probiotic properties that can be explored further using a genomics approach.
Our data on adhesion of LM1 strain showed a significant correlation between adhesion, hydrophobicity of cell surface and autoaggregation. This study gives basic knowledge for the elucidation of the adhesion mechanism of Lactobacillus sp. and prediction of its adherence in specific host models.
在本报告中，我们对黏膜乳杆菌LM1的益生菌潜力进行了表征，重点关注其体外黏附黏蛋白的能力。
采用筛选试验对LM1进行评估。此前已对黏膜乳杆菌属进行过研究，但很少有研究考察该菌种黏附并定殖于肠道黏膜的能力。因此，将LM1的黏附、聚集和病原体抑制试验以及微生物对溶剂的黏附（MATS）试验与另一假定的益生菌约氏乳杆菌PF01和商业菌株鼠李糖乳杆菌GG进行了比较。基于MATS试验，发现这些乳酸杆菌菌株的细胞表面具有疏水性且电子供体能力强，但LM1基因组中预测的表面暴露蛋白的平均亲水性（GRAVY）指数表明大多数蛋白是亲水的。在测试的菌株中，LM1表现出最高的黏附、聚集和疏水性，并显著抑制了大肠杆菌K88和鼠伤寒沙门氏菌KCCM 40253的黏附。观察到黏附、聚集和疏水性之间以及大肠杆菌的共聚集和替代之间的相关性。
由于各种菌株特异性机制，黏附性增加并不总是与病原体抑制作用增强相关。然而，LM1具有有前景的益生菌特性，可使用基因组学方法进一步探索。
我们关于LM1菌株黏附的数据表明黏附、细胞表面疏水性和自聚集之间存在显著相关性。本研究为阐明乳酸杆菌属的黏附机制及其在特定宿主模型中的黏附预测提供了基础知识。
