Miyazawa T, Furuya T, Itagaki S, Tohya Y, Nakano K, Takahashi E, Mikami T
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Arch Virol. 1989;108(1-2):59-68. doi: 10.1007/BF01313743.
Two strains of feline immunodeficiency virus (FIV) were isolated directly from peripheral blood mononuclear cells (PBMCs) of Japanese domestic cats or indirectly from PBMCs of specific pathogen free (SPF) cats inoculated with whole blood from naturally infected cats with FIV by cocultivation with primary PBMCs from SPF cats. Two isolates, designated as FIV TM 1 and FIV TM 2, had a lentivirus-like morphology by electron microscopy, a tropism for interleukin-2 dependent T-lymphocytes and Mg2+-dependent reverse transcriptase activity. By immunoblotting the isolates gave bands at 130, 48, 44, 40, 28, 17, and 13 kDa, and these bands except 130 kDa were detected in FIV Petaluma strain when reacted with the plasma of cats infected naturally with FIV TM 1 strain.
两株猫免疫缺陷病毒（FIV）分别直接从日本家猫的外周血单个核细胞（PBMC）中分离得到，或通过与无特定病原体（SPF）猫的原代PBMC共培养，间接从接种了自然感染FIV猫的全血的SPF猫的PBMC中分离得到。两株分离株，分别命名为FIV TM 1和FIV TM 2，通过电子显微镜观察具有慢病毒样形态，对白细胞介素-2依赖的T淋巴细胞具有嗜性，并具有Mg2+依赖的逆转录酶活性。通过免疫印迹法，分离株在130、48、44、40、28、17和13 kDa处出现条带，当与自然感染FIV TM 1株的猫的血浆反应时，除130 kDa外的这些条带在FIV Petaluma株中也能检测到。
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