猫免疫缺陷病毒的分子克隆
Molecular cloning of feline immunodeficiency virus.
作者信息
Olmsted R A, Barnes A K, Yamamoto J K, Hirsch V M, Purcell R H, Johnson P R
机构信息
Department of Microbiology, Georgetown University, Rockville, MD 20852.
出版信息
Proc Natl Acad Sci U S A. 1989 Apr;86(7):2448-52. doi: 10.1073/pnas.86.7.2448.
Feline immunodeficiency virus (FIV) is a T-lymphotropic retrovirus associated with immunodeficiency and opportunistic infections in cats. The discovery of FIV provides an opportunity for the development of a small animal model for AIDS. To initiate the molecular and biological characterization of FIV, cDNA clones were synthesized and used to isolate a proviral clone of FIV. Molecular cross-hybridization analysis of FIV with five lentiviruses revealed that nucleotide-sequence similarities exist between FIV and these lentiviruses in the gag-pol genes. However, nucleotide-sequence similarities were not seen upon comparison of the FIV long terminal repeat sequence with known viral sequences. Common antigenic determinants appeared to be shared by FIV, caprine arthritis encephalitis virus, and visna virus as shown by serological cross-reactivity of rabbit antibodies to caprine arthritis encephalitis virus and visna virus with the putative FIV core protein p28. These studies demonstrated that FIV is a member of the lentivirus subfamily and is distantly related to the AIDS lentiviruses of primates. Importantly, progeny virions of our molecular clone were infectious for experimentally inoculated cats. The availability of an infectious molecular clone will make possible a detailed dissection of the molecular pathogenesis of FIV, which may facilitate the development of vaccine and therapeutic strategies for AIDS.
猫免疫缺陷病毒（FIV）是一种嗜T淋巴细胞逆转录病毒，与猫的免疫缺陷和机会性感染有关。FIV的发现为开发艾滋病小动物模型提供了契机。为了启动FIV的分子和生物学特性研究，合成了cDNA克隆并用于分离FIV的前病毒克隆。FIV与五种慢病毒的分子交叉杂交分析表明，FIV与这些慢病毒在gag-pol基因上存在核苷酸序列相似性。然而，将FIV长末端重复序列与已知病毒序列进行比较时，未发现核苷酸序列相似性。兔抗山羊关节炎脑炎病毒和维斯纳病毒抗体与推定的FIV核心蛋白p28的血清学交叉反应表明，FIV、山羊关节炎脑炎病毒和维斯纳病毒似乎具有共同的抗原决定簇。这些研究表明，FIV是慢病毒亚科的成员，与灵长类动物的艾滋病慢病毒有远缘关系。重要的是，我们分子克隆的子代病毒颗粒对实验接种的猫具有感染性。有感染性的分子克隆的存在将使详细剖析FIV的分子发病机制成为可能，这可能有助于开发艾滋病疫苗和治疗策略。
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