Mach J P, Forni M, Ritschard J, Buchegger F, Carrel S, Widgren S, Donath A, Alberto P
Oncodev Biol Med. 1980 Aug;1(1):49-69.
Fifty-three patients with histologically proven carcinoma were injected with highly purified [131I]-labeled goat antibodies or fragments of antibodies against carcinoembryonic antigen (CEA). Each patient was tested by external photoscanning 4, 24, 36 and 48 h after injection. In 22 patients (16 of 38 injected with intact antibodies, 5 of 13 with F(ab')2 fragments and 1 of 2 with Fab' fragments), an increased concentration of 131I radioactivity corresponding to the previously known tumor location was detected by photoscanning 36-48 h after injection. Blood pool and secreted radioactivity was determined in all patients by injecting 15 min before scanning, [99mTc]-labeled normal serum albumin and free 99mTc04-. The computerized subtraction of 99mTc from 131I radioactivity enhanced the definition of tumor localization in the 22 positive patients. However, in spite of the computerized subtraction, interpretation of the scans remained doubtful for 12 patients and was entirely negative for 19 additional patients. In order to provide a more objective evaluation for the specificity of the tumor localization of antibodies, 14 patients scheduled for tumor resection were injected simultaneously with [131I]-labeled antibodies or fragments and with [125I]-labeled normal goat IgG or fragments. After surgery, the radioactivity of the two isotopes present either in tumor or adjacent normal tissues was measured in a dual channel scintillation counter. The results showed that the antibodies or their fragments were 2-4 times more concentrated in the tumor than in the normal tissues. In addition, it was shown that the injected antibodies formed immune complexes with circulating CEA and that the amount of immune complexes detectable in serum was roughly proportional to the level of circulating CEA.
53例经组织学证实为癌的患者被注射了高度纯化的[131I]标记的山羊抗癌胚抗原（CEA）抗体或抗体片段。在注射后4、24、36和48小时，对每位患者进行外部光扫描检测。在22例患者中（38例注射完整抗体的患者中有16例，13例注射F(ab')2片段的患者中有5例，2例注射Fab'片段的患者中有1例），在注射后36 - 48小时通过光扫描检测到与先前已知肿瘤位置相对应的131I放射性浓度增加。在所有患者中，在扫描前15分钟注射[99mTc]标记的正常血清白蛋白和游离99mTcO4-来测定血池和分泌的放射性。从131I放射性中计算机化减去99mTc增强了22例阳性患者中肿瘤定位的清晰度。然而，尽管进行了计算机化减法，12例患者的扫描结果解释仍存疑问，另外19例患者的扫描结果完全为阴性。为了对抗体肿瘤定位的特异性提供更客观的评估，14例计划进行肿瘤切除的患者同时被注射了[131I]标记的抗体或片段以及[125I]标记的正常山羊IgG或片段。手术后，在双通道闪烁计数器中测量肿瘤或相邻正常组织中存在的两种同位素的放射性。结果表明，抗体或其片段在肿瘤中的浓度比在正常组织中高2 - 4倍。此外，还表明注射的抗体与循环中的CEA形成免疫复合物，并且血清中可检测到的免疫复合物量大致与循环CEA水平成正比。
