Imperial College, Department of Histopathology, Hammersmith Hospital Campus, London, UK.
Histopathology. 2014 Dec;65(6):749-56. doi: 10.1111/his.12458. Epub 2014 Oct 28.
To characterize the microenvironment of classical Hodgkin lymphoma (cHL) in people living with human immunodeficiency virus (PLWH). The objective was to identify and then quantify the immune cells present in the microenvironment.
Ten samples of cHL from PLWH were compared with 10 samples of cHL from the general population using tissue microarray technology and immunohistochemistry. Sections were immunostained with antibodies for CD30, CD3, CD4, CD8, CD20, CD68R, CD56, CD57, CD123, FoxP3 and granzyme B. A statistically significant reduction of CD4(+) T cells, CD56(+) cells, CD57(+) cells, CD123(+) cells and B cells and an increase in numbers in FoxP3(+) CD8(+) cells was observed in cHL diagnosed in PLWH. No significant differences were seen in the number of CD8(+) T cells, CD4(+) FoxP3(+) T cells and macrophages.
There are considerable differences in the microenvironment of cHL occurring with and without HIV.
描述人类免疫缺陷病毒（PLWH）感染者中经典型霍奇金淋巴瘤（cHL）的微环境。目的是识别并定量微环境中的免疫细胞。
使用组织微阵列技术和免疫组织化学，将 10 例 PLWH 中的 cHL 样本与 10 例普通人群中的 cHL 样本进行比较。使用针对 CD30、CD3、CD4、CD8、CD20、CD68R、CD56、CD57、CD123、FoxP3 和颗粒酶 B 的抗体对切片进行免疫染色。在 PLWH 中诊断出的 cHL 中，观察到 CD4(+)T 细胞、CD56(+)细胞、CD57(+)细胞、CD123(+)细胞和 B 细胞数量显著减少，FoxP3(+)CD8(+)细胞数量增加。CD8(+)T 细胞、CD4(+)FoxP3(+)T 细胞和巨噬细胞的数量没有显著差异。
存在 HIV 伴发和不伴发的 cHL 微环境存在显著差异。
