Alber G, Scheuber P H, Reck B, Sailer-Kramer B, Hartmann A, Hammer D K
Max Planck Institute for Immunobiology, Freiburg, West Germany.
J Allergy Clin Immunol. 1989 Dec;84(6 Pt 1):880-5. doi: 10.1016/0091-6749(89)90383-7.
The staphylococcal enterotoxin B (SEB)-induced immediate-type skin reaction in unsensitized monkeys was used as a nonimmunologic mast cell stimulation to search for possible involvement of local neural mechanisms. Evidence is presented that substance P (SP) plays a predominant role in mediating intradermal SEB challenge in unsensitized monkeys. With a rabbit SP antiserum directed against the C-terminal region of SP, a concentration-dependent inhibition of SEB-induced skin reactivity could be demonstrated. Furthermore, a rabbit antiserum directed against the mast cell activating N-terminal part of SP was capable of impeding SEB-induced skin reactions totally. By use of SP antagonists, significant reduction of skin reactions evoked by SEB was found. Finally, capsaicin pretreatment of the skin caused a substantial inhibition of SEB-induced skin reactivity. These data suggest that SEB exerts its effect on cutaneous mast cells via stimulation of primary sensory neurons that contain SP. Moreover, a new in vivo model is described for studies of nerve-mast cell interactions.
将未致敏的猴子体内由葡萄球菌肠毒素B（SEB）诱导的速发型皮肤反应作为一种非免疫性肥大细胞刺激，以探寻局部神经机制可能发挥的作用。有证据表明，P物质（SP）在介导未致敏猴子皮内SEB激发反应中起主要作用。使用针对SP C末端区域的兔抗SP血清，可证明其对SEB诱导的皮肤反应性具有浓度依赖性抑制作用。此外，针对SP肥大细胞激活N末端部分的兔抗血清能够完全阻止SEB诱导的皮肤反应。通过使用SP拮抗剂，发现SEB诱发的皮肤反应显著降低。最后，皮肤经辣椒素预处理后，SEB诱导的皮肤反应性受到显著抑制。这些数据表明，SEB通过刺激含有SP的初级感觉神经元对皮肤肥大细胞发挥作用。此外，还描述了一种用于研究神经-肥大细胞相互作用的新的体内模型。
