竞争性酶免疫测定法与沉淀抑制试验在分析口腔链球菌多糖抗原决定簇中的比较。

A comparison of competitive enzyme immunoassay and precipitin inhibition tests in the analysis of polysaccharide antigenic determinants of oral streptococci.

作者信息

Ota F, Hirota K, Nagamune H, Senesi S, Satomi Y, Yasuoka M, Akiyama Y, Fukui K, Kawata T

机构信息

Department of Oral Microbiology and Immunology, School of Dentistry, University of Tokushima, Japan.

出版信息

J Immunol Methods. 1989 Nov 30;124(2):259-66. doi: 10.1016/0022-1759(89)90362-1.

DOI:10.1016/0022-1759(89)90362-1

PMID:2480981

Abstract

A rapid and convenient method of competitive enzyme immunoassay (EIA) was explored for the immunological analysis by MAbs of polysaccharide antigens prepared from the mutans group of streptococci, and optimal conditions for it were established. These included the concentrations of antigen, monoclonal antibodies (MAbs) and labelled antibody, temperature, pH and reaction time. The results were compared with those obtained by precipitin inhibition tests using the classical quantitative precipitin reaction, and they were found to be consistent with the latter method. It was concluded that the EIA system can be extended to the immunological study of polysaccharide antigens of oral streptococci using MAbs which perform poorly in precipitation reactions.

摘要

探索了一种快速便捷的竞争性酶免疫测定（EIA）方法，用于由变形链球菌组制备的多糖抗原的单克隆抗体免疫分析，并确定了其最佳条件。这些条件包括抗原、单克隆抗体（MAb）和标记抗体的浓度、温度、pH值和反应时间。将结果与使用经典定量沉淀反应的沉淀抑制试验所得结果进行比较，发现它们与后一种方法一致。得出结论，EIA系统可扩展到使用在沉淀反应中表现不佳的单克隆抗体对口腔链球菌多糖抗原进行免疫研究。