Ota F, Kato H, Hirota K, Inoue H, Tomotake H, Uchiyama C, Minato Y
Department of Food Microbiology, School of Medicine, University of Tokushima, Japan.
FEMS Immunol Med Microbiol. 1994 Mar;8(3):213-7. doi: 10.1111/j.1574-695X.1994.tb00445.x.
A monoclonal antibody (mAb h-448) was prepared after cell fusion of mouse myeloma cells (SP2/0-Ag-14) to the spleen cells of mice immunised with serotype h strain (MF25) of Streptococcus downei. The antibody (IgM class) reacted in enzyme immunoassay only with whole cells as well as purified polysaccharide (PS) antigen of Streptococcus sobrinus (types d and g) and Streptococcus downei (serotype h), but not with cells or purified PS antigen from any other serotypes of the mutans group of streptococci. mAb h-448 also quantitatively precipitated in solution with the purified antigens. Competitive hapten inhibition tests demonstrated that beta-methylgalactopyranoside inhibited the reaction most strongly. Although rhamnose also showed a substantial inhibitory effect, the results of this study indicate that the antigenic determinant of the PS antigen has a structure similar to the beta-methylgalactopyranoside molecule.
将小鼠骨髓瘤细胞（SP2/0-Ag-14）与用唐氏链球菌血清型h菌株（MF25）免疫的小鼠脾细胞进行细胞融合后，制备了一种单克隆抗体（mAb h-448）。该抗体（IgM类）在酶免疫测定中仅与变形链球菌（d型和g型）和唐氏链球菌（血清型h）的全细胞以及纯化的多糖（PS）抗原发生反应，而不与变形链球菌属其他血清型的细胞或纯化的PS抗原发生反应。mAb h-448也能与纯化抗原在溶液中进行定量沉淀。竞争性半抗原抑制试验表明，β-甲基吡喃半乳糖苷对反应的抑制作用最强。虽然鼠李糖也显示出显著的抑制作用，但本研究结果表明，PS抗原的抗原决定簇具有与β-甲基吡喃半乳糖苷分子相似的结构。
