内皮表面层损伤会诱发早期糖尿病大鼠的肾小球超滤。
Injury to the endothelial surface layer induces glomerular hyperfiltration rats with early-stage diabetes.
作者信息
Zhang Chunyang, Meng Yao, Liu Qi, Xuan Miao, Zhang Lanyu, Deng Bo, Zhang Keqin, Liu Zhimin, Lei Tao
机构信息
Division of Endocrinology, Shanghai Tongji Hospital Affiliated Tongji University, No. 389, Xincun Road, Shanghai 200065, China.
Division of Endocrinology, Shanghai Tongji Hospital Affiliated Tongji University, No. 389, Xincun Road, Shanghai 200065, China ; Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.
出版信息
J Diabetes Res. 2014;2014:953740. doi: 10.1155/2014/953740. Epub 2014 Apr 9.
Glomerular endothelial surface layer (ESL) may play a role in the mechanisms of albuminuria in diabetic nephropathy, which lack evidence in vivo. The effects of high glucose on the passage of albumin across the glomerular ESL were analysed in streptozotocin-induced diabetic Sprague-Dawley rats for 4 weeks. Albuminuria and glomerular mesangial matrix were significantly increased in diabetic rats. The passage of albumin across the ESL, as measured by albumin-colloid gold particle density in the glomerular basement membrane (GBM), was increased significantly in diabetic rats. The thickness of the glomerular ESL, examined indirectly by infusing Intralipid into vessels using an electron microscope, was significantly decreased and the GBM exhibited little change in diabetic rats. In summary, the glomerular ESL may play a role in the pathogenesis of albuminuria in rats with early-stage diabetes.
肾小球内皮表面层（ESL）可能在糖尿病肾病蛋白尿的发病机制中起作用，但体内缺乏相关证据。在链脲佐菌素诱导的糖尿病Sprague-Dawley大鼠中，对高糖对白蛋白穿过肾小球ESL的影响进行了为期4周的分析。糖尿病大鼠的蛋白尿和肾小球系膜基质显著增加。通过肾小球基底膜（GBM）中白蛋白 - 胶体金颗粒密度测量，糖尿病大鼠中白蛋白穿过ESL的量显著增加。使用电子显微镜通过向血管内注入脂质乳剂间接检测，糖尿病大鼠的肾小球ESL厚度显著降低，而GBM变化不大。总之，肾小球ESL可能在早期糖尿病大鼠蛋白尿的发病机制中起作用。
Zotero 插件