Yan Xin, Wang Xin-Yue, Sheng Yi-Hua, Zhu Li, Zhang Liang-Deng, Zang Qin
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2014 Apr;34(4):455-9.
To explore the theory of "Fei and Dachang being interior-exteriorly related" and the pathogenesis of lung injury by observing changes of inflammatory cytokines and oxygen free radicals in ulcerative colitis (UC) rats.
Totally 50 healthy male Wistar rats were randomly divided into two groups, the normal control group and the model group, 25 rats in each group. The UC model was established by allergizing colon mucosa combined with TNBS-alcohol (50%) enema. Another 25 rats were recruited as the normal control group. At week 2 and 4 after modeling, the pathomorphological changes of the lung were observed. Furthermore, the contents of tumor necrosis factor alpha (TNF-alpha) and IL-1beta were determined by ELISA. The activities of superoxide dismutase (SOD) and malondialdehyde (MDA) were evaluated with colorimetry.
Compared with the normal control group, the pathomorphology of the lung tissue in the model group appeared abnormal at week 2 and 4. Compared with the normal control group, levels of TNF-alpha, IL-1beta, and MDA in the lung tissue significantly increased in the model group (P < 0. 01) and the activities of SOD significantly decreased (P < 0.05, P < 0.01).
TNF-alpha, IL-1beta, SOD, and MDA might be common material bases for the large intestine involved in lung disease of UC patients, thus providing a modern scientific basis for the theory of Fei and Dachang being interior-exteriorly related.
通过观察溃疡性结肠炎（UC）大鼠炎症细胞因子和氧自由基的变化，探讨“肺与大肠相表里”理论及肺损伤的发病机制。
将50只健康雄性Wistar大鼠随机分为两组，即正常对照组和模型组，每组25只。采用结肠黏膜致敏联合TNBS - 乙醇（50%）灌肠法建立UC模型。另取25只大鼠作为正常对照组。造模后第2周和第4周，观察肺组织的病理形态学变化。此外，采用ELISA法检测肿瘤坏死因子α（TNF - α）和IL - 1β的含量。用比色法评估超氧化物歧化酶（SOD）和丙二醛（MDA）的活性。
与正常对照组相比，模型组肺组织病理形态在第2周和第4周出现异常。与正常对照组相比，模型组肺组织中TNF - α、IL - 1β和MDA水平显著升高（P < 0.01），SOD活性显著降低（P < 0.05，P < 0.01）。
TNF - α、IL - 1β、SOD和MDA可能是UC患者大肠病及肺的共同物质基础，从而为“肺与大肠相表里”理论提供现代科学依据。
