Lai Ji-Ching, Hong Chin-Yih
Graduate Institute of Biomedical Engineering, National Chung Hsing University , Taichung, 402 Taiwan, R.O.C.
ACS Comb Sci. 2014 Jul 14;16(7):321-7. doi: 10.1021/co5000272. Epub 2014 May 21.
A new SELEX protocol for the development of DNA aptamers has been demonstrated, referred to as magnetic-assisted rapid aptamer selection (MARAS). This method uses magnetic beads and an externally applied rotating magnetic field to provide the competitive mechanism for the selection aptamers with different affinities to the molecular target. The MARAS protocol efficiently generated aptamers with high affinity and specificity for C-reactive protein, a common cardiovascular disease indicator. The binding affinities of the selected aptamers could be varied by changing the frequency of the externally applied rotating magnetic field and optimal cases bound with low-nanomolar dissociation constants.
一种用于开发DNA适配体的新型SELEX方案已得到验证，即磁辅助快速适配体筛选（MARAS）。该方法使用磁珠和外部施加的旋转磁场，为筛选对分子靶标具有不同亲和力的适配体提供竞争机制。MARAS方案有效地产生了对常见心血管疾病指标C反应蛋白具有高亲和力和特异性的适配体。通过改变外部施加的旋转磁场的频率，可以改变所选适配体的结合亲和力，最佳情况下结合的解离常数为低纳摩尔。
