Wang P, Duan D, Zhou X, Li X, Yang J, Deng M, Xu Y
State Key Laboratory of Oral Diseases, Chengdu, China; Department of Applied Oral Sciences, Center for Periodontology, The Forsyth Institute, Cambridge, MA, USA.
J Periodontal Res. 2015 Feb;50(1):113-22. doi: 10.1111/jre.12187. Epub 2014 May 12.
Human beta-defensins (hBDs) are a group of antimicrobial peptides important in epithelial innate immunity, and their differential expression is associated with periodontal diseases. The aim of this study was to explore relationships among hBDs, total subgingival bacteria and periodontopathogens in healthy subjects and in patients with chronic periodontitis.
The periodontal clinical parameters of 29 healthy subjects and 25 patients with chronic periodontitis were recorded. The relative expression of hBD1, hBD2 and hBD3 genes in gingival biopsies was measured using real-time PCR. The numbers of total bacteria and of Treponema denticola, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Fusobacterium nucleatum and Tannerella forsythia in subgingival plaque were quantified by real-time PCR. Data were analyzed using the Mann-Whitney U-test and Spearman's rank correlation test.
No significant differences in expression of the hBD genes were found between the group of healthy subjects and the group of patients with chronic periodontitis. Total bacteria and T. denticola were detected in all participants. F. nucleatum and T. forsythia were detected in all patients with chronic periodontitis and in 86.21% and 51.72%, respectively, of healthy volunteers. P. gingivalis and A. actinomycetemcomitans were detected in 24.14% and 17.24%, respectively, of the healthy group and in 84.00% and 12.00%, respectively, of the chronic periodontitis group. The prevalence of all bacteria, except A. actinomycetemcomitans, was significantly higher in the group of patients with chronic periodontitis than in the group of healthy subjects (p < 0.05). A significant, negative correlation was observed between total bacteria and hBD-2 (r = -0.384, p = 0.011). Upon analyzing the data in different groups, total bacteria and hBD-2 were significantly correlated (r = -0.492, p = 0.026) only in the group of healthy subjects.
The negative correlations between hBD-2 and total bacteria, especially in the group of healthy subjects, indicate that hBDs may play an important role by limiting an increase of bacterial load at the initial stage of periodontitis.
人β-防御素（hBDs）是一组在上皮固有免疫中起重要作用的抗菌肽，其差异表达与牙周疾病相关。本研究旨在探讨健康受试者和慢性牙周炎患者中hBDs、龈下细菌总数与牙周病原菌之间的关系。
记录29名健康受试者和25名慢性牙周炎患者的牙周临床参数。采用实时荧光定量PCR检测牙龈活检组织中hBD1、hBD2和hBD3基因的相对表达。通过实时荧光定量PCR对龈下菌斑中细菌总数以及具核梭杆菌、伴放线聚集杆菌、牙龈卟啉单胞菌、具核梭杆菌和福赛坦纳菌的数量进行定量。数据采用Mann-Whitney U检验和Spearman等级相关检验进行分析。
健康受试者组和慢性牙周炎患者组之间hBD基因表达无显著差异。所有受试者均检测到细菌总数和具核梭杆菌。所有慢性牙周炎患者以及分别86.21%和51.72%的健康志愿者检测到具核梭杆菌和福赛坦纳菌。健康组中牙龈卟啉单胞菌和伴放线聚集杆菌的检出率分别为24.14%和17.24%，慢性牙周炎组中分别为84.00%和12.00%。除伴放线聚集杆菌外，所有细菌在慢性牙周炎患者组中的患病率均显著高于健康受试者组（p < 0.05）。细菌总数与hBD-2之间存在显著的负相关（r = -0.384，p = 0.011）。在不同组中分析数据时，仅在健康受试者组中细菌总数与hBD-2显著相关（r = -0.492，p = 0.026）。
hBD-2与细菌总数之间的负相关，尤其是在健康受试者组中，表明hBDs可能通过在牙周炎初期限制细菌载量的增加发挥重要作用。
