Smith R E, Kister S E, Carozzi N B
Corporate Molecular Biology, Abbott Laboratories, Abbott Park, IL 60064.
Gene. 1989 Jul 15;79(2):239-48. doi: 10.1016/0378-1119(89)90206-0.
The major inner capsid protein (VP6) of SA-11 simian rotavirus has been expressed in Escherichia coli using a cloned cDNA derived from SA-11 double-stranded RNA segment 6. The cloned gene was fused to the N-terminal coding sequence of lacZ resulting in the synthesis of a 44-kDa protein. Several smaller polypeptides were also observed, resulting predominantly from transcription and translation within the gene 6 coding sequence. The recombinant VP6 proved to be antigenic by immunoblot analysis using polyclonal serum against SA-11 rotavirus and by Western-blot analysis using monospecific serum derived from purified viral VP6.
