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采用氢核磁共振代谢组学方法研究乙酰半胱氨酸对慢性高原病大鼠的影响及机制。

Effects and mechanisms of acetyl-L-cysteine in rats with chronic mountain sickness with H1-NMR metabolomics methods.

作者信息

Maimaitiyimin Dilinuer, Aikemu Ainiwaer, Kamilijiang Mayila, Salamu Adila, Zhang Xiangyang

机构信息

Department of Cardiology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, China (mainland).

Department of Drug Analysis, Xinjiang Medical University, Urumqi, China (mainland).

出版信息

Med Sci Monit. 2014 May 10;20:767-73. doi: 10.12659/MSM.890244.

DOI:10.12659/MSM.890244
PMID:24816079
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4026147/
Abstract

BACKGROUND

We established a rat model of chronic mountain sickness using acetyl-L-cysteine. Then we studied the effects and mechanisms of acetyl-L-cysteine (Da) in rats with chronic mountain sickness using nuclear magnetic resonance (H1-NMR) metabolomics methods.

MATERIAL AND METHODS

Using NMR spectroscopy combined with pattern recognition and orthogonal partial least squares discriminant analysis, we analyzed the impact of Da on blood metabolism in rats with chronic mountain sickness by determining different metabolites and changes in metabolic network in the blood of rats with mountain sickness after the intragastric administration of different doses of Da suspension.

RESULTS

Increased levels of amino acids (valine, tyrosine, 1-methyl-histidine, leucine, phenylalanine, and methionine) were detected in the blood of rats in the chronic mountain sickness group, yet significantly decreased levels were detected in control rats. At the same time, β-glucose and α-glucose levels were markedly elevated in the blood of rats in the model group but decreased in the chronic mountain sickness group, which indicated a statistically significant difference compared with the chronic altitude sickness model group (P<0.05).

CONCLUSIONS

Da has a significant impact on the metabolism of rats with chronic mountain sickness. Da may act on the disturbed glucose metabolism and amino acid metabolism in rats triggered by chronic mountain sickness, resulting in the treatment and prevention of this disease.

摘要

背景

我们使用乙酰半胱氨酸建立了慢性高原病大鼠模型。然后，我们使用核磁共振（H1-NMR）代谢组学方法研究了乙酰半胱氨酸（Da）对慢性高原病大鼠的影响及其机制。

材料与方法

采用核磁共振光谱结合模式识别和正交偏最小二乘法判别分析，通过测定不同剂量Da混悬液灌胃后慢性高原病大鼠血液中不同代谢物及代谢网络变化，分析Da对慢性高原病大鼠血液代谢的影响。

结果

慢性高原病组大鼠血液中氨基酸（缬氨酸、酪氨酸、1-甲基组氨酸、亮氨酸、苯丙氨酸和蛋氨酸）水平升高，而对照组大鼠血液中氨基酸水平显著降低。同时，模型组大鼠血液中β-葡萄糖和α-葡萄糖水平显著升高，而慢性高原病组大鼠血液中β-葡萄糖和α-葡萄糖水平降低，与慢性高原病模型组相比差异有统计学意义（P<0.05）。

结论

Da对慢性高原病大鼠的代谢有显著影响。Da可能作用于慢性高原病引发大鼠的糖代谢和氨基酸代谢紊乱，从而对该病起到治疗和预防作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c59c/4026147/9b732a8708d6/medscimonit-20-767-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c59c/4026147/a955906f49a0/medscimonit-20-767-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c59c/4026147/9b732a8708d6/medscimonit-20-767-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c59c/4026147/a955906f49a0/medscimonit-20-767-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c59c/4026147/9b732a8708d6/medscimonit-20-767-g002.jpg

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