基因转移：转化/电穿孔法。

Gene transfer: transformation/electroporation.

作者信息

Cadoret Frédéric, Soscia Chantal, Voulhoux Romé

机构信息

Laboratoire d'Ingénierie des Systèmes Macromoléculaires, UMR7255 - CNRS - Aix Marseille Université - IMM, 31 chemin Joseph Aiguier, 13402, Cedex 20, France.

出版信息

Methods Mol Biol. 2014;1149:11-5. doi: 10.1007/978-1-4939-0473-0_2.

DOI:10.1007/978-1-4939-0473-0_2

PMID:24818892

Abstract

Since Pseudomonas aeruginosa is a non-naturally competent bacterium, various methods have been developed to transfer exogenous DNA. Alternatively to transduction and conjugation, electroporation can also be used to transfer exogenous DNA molecules into Pseudomonas. Electroporation uses an electric field which generates pores in bacterial membranes allowing the entry of the exogenous DNA molecule. In contrast to conjugation which is restricted to the transfer of DNA from one bacterial cell to another, electroporation can be used to transfer all types of DNA resuspended in water.

摘要

由于铜绿假单胞菌是一种非天然感受态细菌，人们已开发出多种方法来转移外源DNA。除了转导和接合之外，电穿孔也可用于将外源DNA分子导入铜绿假单胞菌。电穿孔利用电场在细菌膜上产生孔隙，使外源DNA分子得以进入。与仅限于将DNA从一个细菌细胞转移到另一个细菌细胞的接合不同电穿孔可用于转移溶解在水中的所有类型的DNA。