Tian Peng, Xu Bo, Sun Hanxiao, Li Xiuying, Li Zhi, Wei Pijin
Institute of Genomic Medicine, College of Pharmacy, Jinan University, Guangzhou 510632, China.
Institute of Genomic Medicine, College of Pharmacy, Jinan University, Guangzhou 510632, China.
Diagn Microbiol Infect Dis. 2014 Aug;79(4):405-12. doi: 10.1016/j.diagmicrobio.2014.04.002. Epub 2014 Apr 13.
Antibiotic-resistant probiotics may be advantageous for antibiotic-induced gut microbiota imbalance. In this article, we aimed to isolate antibiotic-resistant bacteria as potential probiotics. Feces from 3 healthy adults and 2 infants were used to isolate the antibiotic-resistant bacteria. Then we established gut microbiota imbalance mice model by antibiotics treatment and used it to assess the effect of the probiotics. Finally, we identified 8 isolates, and 6 of them were used as probiotics cocktail. Number of anaerobe, lactobacilli, and Bifidobacterium in feces were higher in the probiotic group (9.47±0.35 log10CFU/g, 8.74±0.18 log10CFU/g, 7.24±0.38 log10CFU/g, respectively) compared with model group (P<0.05). Richness and diversity index of probiotic group (19.79±0.29 and 2.95±0.06, respectively) were larger than model group (P<0.05). Diarrhea and mucosal edema had been alleviated during probiotic treatment. Our results validated that bacteriotherapy was available to treat gut microbiota imbalance.
抗生素抗性益生菌可能对抗生素引起的肠道微生物群失衡具有优势。在本文中，我们旨在分离抗生素抗性细菌作为潜在的益生菌。使用3名健康成年人和2名婴儿的粪便来分离抗生素抗性细菌。然后我们通过抗生素治疗建立肠道微生物群失衡小鼠模型，并用于评估益生菌的效果。最后，我们鉴定出8株分离菌，其中6株用作益生菌混合物。与模型组相比，益生菌组粪便中厌氧菌、乳酸菌和双歧杆菌的数量更高（分别为9.47±0.35 log10CFU/g、8.74±0.18 log10CFU/g、7.24±0.38 log10CFU/g）（P<0.05）。益生菌组的丰富度和多样性指数（分别为19.79±0.29和2.95±0.06）大于模型组（P<0.05）。在益生菌治疗期间，腹泻和粘膜水肿得到缓解。我们的结果证实了细菌疗法可用于治疗肠道微生物群失衡。
