利用改良的 Mos1 转座子在秀丽隐杆线虫中进行随机和靶向的转基因插入。
Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon.
机构信息
1] Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah, USA. [2] Department of Biology, University of Utah, Salt Lake City, Utah, USA. [3] Danish National Research Foundation Centre for Cardiac Arrhythmia, University of Copenhagen, Copenhagen, Denmark.
1] Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah, USA. [2] Department of Biology, University of Utah, Salt Lake City, Utah, USA.
出版信息
Nat Methods. 2014 May;11(5):529-34. doi: 10.1038/nmeth.2889. Epub 2014 Mar 16.
We have generated a recombinant Mos1 transposon that can insert up to 45-kb transgenes into the Caenorhabditis elegans genome. The minimal Mos1 transposon (miniMos) is 550 bp long and inserts DNA into the genome at high frequency (~60% of injected animals). Genetic and antibiotic markers can be used for selection, and the transposon is active in C. elegans isolates and Caenorhabditis briggsae. We used the miniMos transposon to generate six universal Mos1-mediated single-copy insertion (mosSCI) landing sites that allow targeted transgene insertion with a single targeting vector into permissive expression sites on all autosomes. We also generated two collections of strains: a set of bright fluorescent insertions that are useful as dominant, genetic balancers and a set of lacO insertions to track genome position.
我们已经构建了一种重组 Mos1 转座子，它可以将长达 45kb 的转基因插入秀丽隐杆线虫的基因组。最小 Mos1 转座子（miniMos）长 550bp，能以较高的频率（约 60%的注射动物）将 DNA 插入基因组。遗传和抗生素标记物可用于选择，该转座子在秀丽隐杆线虫分离株和 Caenorhabditis briggsae 中具有活性。我们使用 miniMos 转座子生成了六个通用的 Mos1 介导的单拷贝插入（mosSCI）着陆点，这些着陆点允许使用单个靶向载体将转基因插入到所有常染色体的允许表达位点。我们还生成了两个品系集合：一套明亮的荧光插入，可用作显性遗传平衡器，以及一套 lacO 插入，用于跟踪基因组位置。
Zotero 插件