Centre de Génétique et de Physiologie Moléculaires et Cellulaires, CNRS UMR 5534, Campus de la Doua, Villeurbanne, France.
PLoS One. 2012;7(2):e30482. doi: 10.1371/journal.pone.0030482. Epub 2012 Feb 8.
Methods that use homologous recombination to engineer the genome of C. elegans commonly use strains carrying specific insertions of the heterologous transposon Mos1. A large collection of known Mos1 insertion alleles would therefore be of general interest to the C. elegans research community. We describe here the optimization of a semi-automated methodology for the construction of a substantial collection of Mos1 insertion mutant strains. At peak production, more than 5,000 strains were generated per month. These strains were then subject to molecular analysis, and more than 13,300 Mos1 insertions characterized. In addition to targeting directly more than 4,700 genes, these alleles represent the potential starting point for the engineered deletion of essentially all C. elegans genes and the modification of more than 40% of them. This collection of mutants, generated under the auspices of the European NEMAGENETAG consortium, is publicly available and represents an important research resource.
方法，使用同源重组来设计的基因组线虫通常使用特定的插入的异体转座子 Mos1。一个大的集合的已知 Mos1 插入等位基因因此将是一般利益的线虫研究界。我们在这里描述的优化的半自动方法来构建一个实质性的收集 Mos1 插入突变株。在生产高峰期，每月生成超过 5000 株。然后这些菌株进行分子分析，并超过 13300 Mos1 插入特征。除了直接针对超过 4700 个基因，这些等位基因代表潜在的起点为设计删除基本上所有的线虫基因和修改超过 40%的他们。这个集合的突变体，产生的主持下的欧洲 NEMAGENETAG 联盟，是公开的，是一个重要的研究资源。
