Tillet Y, Caldani M, Batailler M
Laboratoire de Neuroendocrinologie, INRA, Nouzilly, France.
J Chem Neuroanat. 1989 Nov-Dec;2(6):319-26.
Immunohistochemical double-labellings of luteinizing hormone-releasing hormone and neuropeptide Y, serotonin, tyrosine hydroxylase and dopamine-beta-hydroxylase, were performed in the preoptic area at the level of the organum vasculosum laminae terminalis. The observed neuropeptide Y-, serotonin-, tyrosine hydroxylase- and dopamine-beta-hydroxylase-immunoreactive fibres presented large varicosities when they were found within close proximity to luteinizing hormone-releasing hormone-containing perikarya. The fact that neuropeptide Y- and dopamine-beta-hydroxylase-immunoreactive fibres exhibited the same morphological characteristics and the comparison of the distribution of these two fibre populations raised the possibility of the co-localization of neuropeptide Y and dopamine-beta-hydroxylase in the same fibres. Dopamine-beta-hydroxylase- and tyrosine hydroxylase-immunoreactive fibres were morphologically different, suggesting that both dopaminergic and noradrenergic fibres could contact luteinizing hormone-releasing hormone-containing perikarya. Serotonin-immunoreactive fibres were also found close to the perikarya and to the proximal dendrite of the luteinizing hormone-releasing hormone-containing neurons. This study showed only putative sites of interactions between chemically identified fibres and luteinizing hormone-releasing hormone-containing neurons. Further ultrastructural immunocytochemical investigations are needed to ascertain the existence of synaptic contacts.
在终板血管器水平的视前区进行了促黄体生成素释放激素与神经肽Y、5-羟色胺、酪氨酸羟化酶和多巴胺β-羟化酶的免疫组织化学双标记。当观察到神经肽Y、5-羟色胺、酪氨酸羟化酶和多巴胺β-羟化酶免疫反应性纤维靠近含促黄体生成素释放激素的核周体时，呈现出大的膨体。神经肽Y和多巴胺β-羟化酶免疫反应性纤维表现出相同的形态特征，以及这两种纤维群体分布的比较，增加了神经肽Y和多巴胺β-羟化酶在同一纤维中共定位的可能性。多巴胺β-羟化酶和酪氨酸羟化酶免疫反应性纤维在形态上不同，表明多巴胺能纤维和去甲肾上腺素能纤维都可以与含促黄体生成素释放激素的核周体接触。5-羟色胺免疫反应性纤维也在含促黄体生成素释放激素神经元的核周体和近端树突附近被发现。这项研究仅显示了化学鉴定的纤维与含促黄体生成素释放激素神经元之间相互作用的假定部位。需要进一步的超微结构免疫细胞化学研究来确定突触接触的存在。
