Tillet Y, Batailler M, Thibault J
Unité de Neuroendocrinologie Sexuelle, INRA Station de Physiologie de la Reproduction, Nouzilly, France.
J Comp Neurol. 1993 Apr 8;330(2):195-220. doi: 10.1002/cne.903300205.
The preoptic area contains most of the luteinizing hormone releasing hormone immunoreactive neurons and numerous monoaminergic afferents whose cell origins are unknown in sheep. Using tract tracing methods with a specific retrograde fluorescent tracer, fluorogold, we examined the cells of origin of afferents to the medial preoptic area in sheep. Among the retrogradely labeled neurons, immunohistochemistry for tyrosine hydroxylase, dopamine-beta-hydroxylase, phenylethanolamine N-methyltransferase, and serotonin was used to characterize catecholamine and serotonin fluorogold labeled neurons. Most of the afferents came from the ipsilateral side to the injection site. It was observed that the medial preoptic area received major inputs from the diagonal band of Broca, the lateral septum, the thalamic paraventricular nucleus, the lateral hypothalamus, the area dorsolateral to the third ventricle, the perimamillary area, the amygdala, and the ventral part of the hippocampus. Other numerous, scattered, retrogradely labeled neurons were observed in the ventral part of the preoptic area, the vascular organ of the lamina terminalis, the ventromedial part of the hypothalamus, the periventricular area, the area lateral to the interpeduncular nucleus, and the dorsal vagal complex. Noradrenergic afferents came from the complex of the locus coeruleus (A6/A7 groups) and from the ventro-lateral medulla (group A1). However, dopaminergic and adrenergic neuronal groups retrogradely labeled with fluorogold were not observed. Serotoninergic fluorogold labeled neurons belonged to the medial raphe nucleus (B8, B5) and to the serotoninergic group situated lateral to the interpeduncular nucleus (S4). In the light of these anatomical data we hypothesize that these afferents have a role in the regulation of several functions of the preoptic area, particularly those related to reproduction. Accordingly these afferents could be involved in the control of luteinizing hormone releasing hormone (LHRH) pulsatility or of preovulatory LHRH surge.
视前区包含大多数促黄体生成素释放激素免疫反应性神经元以及众多单胺能传入神经，在绵羊中，这些传入神经的细胞起源尚不清楚。我们使用特异性逆行荧光示踪剂氟金，通过束路追踪方法，研究了绵羊视前内侧区传入神经的细胞起源。在逆行标记的神经元中，利用酪氨酸羟化酶、多巴胺-β-羟化酶、苯乙醇胺N-甲基转移酶和5-羟色胺的免疫组织化学方法，对儿茶酚胺和5-羟色胺氟金标记的神经元进行特征描述。大多数传入神经来自注射部位的同侧。研究发现，视前内侧区主要接受来自布罗卡斜带、外侧隔、丘脑室旁核、外侧下丘脑、第三脑室背外侧区、乳头周区、杏仁核以及海马腹侧部分的输入。在视前区腹侧、终板血管器、下丘脑腹内侧部分、室周区、脚间核外侧区以及迷走背侧复合体中，还观察到其他众多分散的逆行标记神经元。去甲肾上腺素能传入神经来自蓝斑复合体（A6/A7组）和延髓腹外侧（A1组）。然而，未观察到被氟金逆行标记的多巴胺能和肾上腺素能神经元群。5-羟色胺能氟金标记的神经元属于中缝内侧核（B8、B5）以及位于脚间核外侧的5-羟色胺能神经元群（S4）。根据这些解剖学数据，我们推测这些传入神经在视前区多种功能的调节中发挥作用，尤其是那些与生殖相关的功能。因此，这些传入神经可能参与促黄体生成素释放激素（LHRH）脉冲性分泌或排卵前LHRH峰的控制。
