Böttger E C
Medizinische Hochschule Hannover, Institut für Medizinische Mikrobiologie, F.R.G.
FEMS Microbiol Lett. 1989 Nov;53(1-2):171-6. doi: 10.1016/0378-1097(89)90386-8.
Ribosomal RNA sequences are an appealing target for bacterial classification as well as for development of group- or species-specific DNA probes. Using the polymerase chain reaction and synthetic primers, the feasibility of this gene amplification technique for rapid sequence determination of the major 16S ribosomal RNA domains from small amounts of input DNA is demonstrated. Information useful for phylogenetic classification as well as for construction of specific DNA probes may be obtained by comparison with known sequences.
核糖体RNA序列是细菌分类以及开发组特异性或种特异性DNA探针的一个有吸引力的靶点。利用聚合酶链反应和合成引物，证明了这种基因扩增技术可用于从少量输入DNA快速测定主要16S核糖体RNA结构域的序列。通过与已知序列进行比较，可获得有助于系统发育分类以及构建特异性DNA探针的信息。
