Rogall T, Flohr T, Böttger E C
Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, FRG.
J Gen Microbiol. 1990 Sep;136(9):1915-20. doi: 10.1099/00221287-136-9-1915.
Nucleotide sequences specific for a range of Mycobacterium species were defined by computer-assisted sequence comparisons of small subunit ribosomal RNA. A polymerase chain reaction-based sequencing strategy was used to demonstrate that the 16S rRNA sequence can be used for the rapid identification of mycobacterial isolates. Identification at the species level can be obtained within 2 d, requiring less than 10,000 bacteria. This procedure reliably differentiates Mycobacterium spp. which are difficult to identify by classical methods, such as M. malmoense, M. szulgai and M. flavescens.
通过对小亚基核糖体RNA进行计算机辅助序列比较，确定了一系列分枝杆菌属物种特有的核苷酸序列。基于聚合酶链反应的测序策略被用于证明16S rRNA序列可用于快速鉴定分枝杆菌分离株。在2天内即可实现种水平的鉴定，所需细菌数量少于10000个。该方法能可靠地区分用传统方法难以鉴定的分枝杆菌属物种，如马尔默分枝杆菌、苏尔加分枝杆菌和淡黄分枝杆菌。
