通过聚合酶链反应扩增高变16S rRNA基因启动子区域快速鉴定分枝杆菌菌种
Rapid identification of mycobacterial species by PCR amplification of hypervariable 16S rRNA gene promoter region.
作者信息
Dobner P, Feldmann K, Rifai M, Löscher T, Rinder H
机构信息
Department for Infectious Diseases and Tropical Medicine, University of Munich, Germany.
出版信息
J Clin Microbiol. 1996 Apr;34(4):866-9. doi: 10.1128/jcm.34.4.866-869.1996.
Abstract
A total of 0.3 to 0.4 kb of the promoter region of the 16S rRNA genes from Mycobacterium tuberculosis, M. gordonae, M. xenopi, and M. leprae was PCR amplified, cloned, and sequenced. The observed number of substitutions, insertions, and deletions exceeded those found in previously used target sequences, including the entire 16S coding region. A simple and generally applicable restriction fragment length polymorphism method that can be used to distinguish between mycobacterial species is described.
摘要
对来自结核分枝杆菌、戈登分枝杆菌、偶发分枝杆菌和麻风分枝杆菌的16S rRNA基因启动子区域总共0.3至0.4 kb进行了PCR扩增、克隆和测序。观察到的替换、插入和缺失数量超过了先前使用的靶序列（包括整个16S编码区域）中的数量。本文描述了一种可用于区分分枝杆菌菌种的简单且普遍适用的限制性片段长度多态性方法。
相似文献
1
Rapid identification of mycobacterial species by PCR amplification of hypervariable 16S rRNA gene promoter region.通过聚合酶链反应扩增高变16S rRNA基因启动子区域快速鉴定分枝杆菌菌种
J Clin Microbiol. 1996 Apr;34(4):866-9. doi: 10.1128/jcm.34.4.866-869.1996.
2
Novel diagnostic algorithm for identification of mycobacteria using genus-specific amplification of the 16S-23S rRNA gene spacer and restriction endonucleases.使用16S - 23S rRNA基因间隔区的属特异性扩增和限制性内切酶鉴定分枝杆菌的新型诊断算法。
J Clin Microbiol. 2000 Mar;38(3):1094-104. doi: 10.1128/JCM.38.3.1094-1104.2000.
3
Nucleotide sequence and secondary structures of precursor 16S rRNA of slow-growing mycobacteria.缓慢生长分枝杆菌前体16S rRNA的核苷酸序列和二级结构
Microbiology (Reading). 1994 Jan;140 ( Pt 1):123-32. doi: 10.1099/13500872-140-1-123.
4
Diversity of the 32-kilodalton protein gene may form a basis for species determination of potentially pathogenic mycobacterial species.32千道尔顿蛋白基因的多样性可能为潜在致病性分枝杆菌物种的鉴定提供依据。
J Clin Microbiol. 1997 Mar;35(3):769-73. doi: 10.1128/jcm.35.3.769-773.1997.
5
Identification of mycobacteria from animals by restriction enzyme analysis and direct DNA cycle sequencing of polymerase chain reaction-amplified 16S rRNA gene sequences.通过限制性内切酶分析以及聚合酶链反应扩增的16S rRNA基因序列的直接DNA循环测序从动物中鉴定分枝杆菌。
J Clin Microbiol. 1993 Dec;31(12):3216-22. doi: 10.1128/jcm.31.12.3216-3222.1993.
6
Evaluation of the LiPA MYCOBACTERIA assay for identification of mycobacterial species from BACTEC 12B bottles.评估LiPA分枝杆菌检测法用于从BACTEC 12B瓶中鉴定分枝杆菌菌种的效果。
J Clin Microbiol. 2000 May;38(5):1915-9. doi: 10.1128/JCM.38.5.1915-1919.2000.
7
The nucleotide sequence of the promoter, 16S rRNA and spacer region of the ribosomal RNA operon of Mycobacterium tuberculosis and comparison with Mycobacterium leprae precursor rRNA.结核分枝杆菌核糖体RNA操纵子的启动子、16S rRNA和间隔区的核苷酸序列及其与麻风分枝杆菌前体rRNA的比较。
J Gen Microbiol. 1992 Aug;138 Pt 8:1717-27. doi: 10.1099/00221287-138-8-1717.
8
PCR assay based on DNA coding for 16S rRNA for detection and identification of mycobacteria in clinical samples.基于编码16S核糖体RNA的DNA的聚合酶链反应检测法，用于临床样本中分枝杆菌的检测与鉴定。
J Clin Microbiol. 1995 Dec;33(12):3225-33. doi: 10.1128/jcm.33.12.3225-3233.1995.
9
Rapid and sensitive method for the detection of Mycobacterium chlorophenolicum PCP-1 in soil based on 16S rRNA gene-targeted PCR.基于16S rRNA基因靶向PCR的土壤中氯酚分枝杆菌PCP-1快速灵敏检测方法
Appl Environ Microbiol. 1996 Apr;62(4):1478-80. doi: 10.1128/aem.62.4.1478-1480.1996.
10
Histologic and genotypic characterization of a novel Mycobacterium species found in three cats.在三只猫中发现的一种新型分枝杆菌的组织学和基因型特征
J Clin Microbiol. 2002 Jul;40(7):2425-30. doi: 10.1128/JCM.40.7.2425-2430.2002.
引用本文的文献
1
Identification and Characterization of Mycobacterial Species Using Whole-Genome Sequences.基于全基因组序列鉴定和描述分枝杆菌菌种。
Methods Mol Biol. 2021;2314:399-457. doi: 10.1007/978-1-0716-1460-0_19.
2
Identification of nontuberculous mycobacteria using multilocous sequence analysis of 16S rRNA, hsp65, and rpoB.使用16S rRNA、hsp65和rpoB的多位点序列分析鉴定非结核分枝杆菌。
J Clin Lab Anal. 2018 Jan;32(1). doi: 10.1002/jcla.22184. Epub 2017 Feb 23.
3
Diagnostic value of PCR in genitourinary tuberculosis.聚合酶链反应在泌尿生殖系统结核中的诊断价值
Indian J Clin Biochem. 2013 Jul;28(3):305-8. doi: 10.1007/s12291-012-0279-7. Epub 2012 Dec 6.
4
A simple and efficient multiplex PCR assay for the identification of Mycobacterium genus and Mycobacterium tuberculosis complex to the species level.一种简单而高效的多重 PCR 检测方法，可用于鉴定分枝杆菌属和结核分枝杆菌复合体到种的水平。
Yonsei Med J. 2013 Sep;54(5):1220-6. doi: 10.3349/ymj.2013.54.5.1220.
5
Development of a real-time qPCR method for detection and enumeration of Mycobacterium spp. in surface water.建立一种实时 qPCR 方法，用于检测和计数地表水中的分枝杆菌属。
Appl Environ Microbiol. 2010 Nov;76(21):7348-51. doi: 10.1128/AEM.00942-10. Epub 2010 Sep 17.
6
Computational identification of composite regulatory sites in 16s-rRNA gene promoters of Mycobacterium species.分枝杆菌属16S - rRNA基因启动子中复合调控位点的计算识别
Bioinformation. 2008 Jun 16;2(8):363-8. doi: 10.6026/97320630002363.
7
Computational approach involving use of the internal transcribed spacer 1 region for identification of Mycobacterium species.利用内部转录间隔区1区域鉴定分枝杆菌属物种的计算方法。
J Clin Microbiol. 2005 Aug;43(8):3811-7. doi: 10.1128/JCM.43.8.3811-3817.2005.
8
Evaluation of amplified rDNA restriction analysis (ARDRA) for the identification of cultured mycobacteria in a diagnostic laboratory.评估扩增核糖体DNA限制酶切分析（ARDRA）在诊断实验室中用于鉴定培养分枝杆菌的应用。
BMC Microbiol. 2002 Mar 1;2:4. doi: 10.1186/1471-2180-2-4.
9
Rapid identification of Mycobacteria to the species level using INNO-LiPA Mycobacteria, a reverse hybridization assay.使用INNO-LiPA分枝杆菌（一种反向杂交检测法）对分枝杆菌进行快速种水平鉴定。
J Clin Microbiol. 2001 Dec;39(12):4477-82. doi: 10.1128/JCM.39.12.4477-4482.2001.
本文引用的文献
1
Mycobacterium intermedium sp. nov.中间分枝杆菌，新种
Int J Syst Bacteriol. 1993 Apr;43(2):204-9. doi: 10.1099/00207713-43-2-204.
2
Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis.通过聚合酶链反应和限制性酶切分析快速鉴定分枝杆菌至种水平
J Clin Microbiol. 1993 Feb;31(2):175-8. doi: 10.1128/jcm.31.2.175-178.1993.
3
Nucleic acid sequence-based amplification (NASBA) for the identification of mycobacteria.基于核酸序列扩增技术（NASBA）用于分枝杆菌鉴定
J Gen Microbiol. 1993 Oct;139(10):2423-9. doi: 10.1099/00221287-139-10-2423.
4
Mycobacterium genavense sp. nov.日内瓦分枝杆菌新种
Int J Syst Bacteriol. 1993 Oct;43(4):841-3. doi: 10.1099/00207713-43-4-841.
5
6
Cervical lymphadenitis in a child caused by a previously unknown mycobacterium.一名儿童的颈部淋巴结炎由一种此前未知的分枝杆菌引起。
J Infect Dis. 1993 Jan;167(1):237-40. doi: 10.1093/infdis/167.1.237.
7
Nucleotide sequence and secondary structures of precursor 16S rRNA of slow-growing mycobacteria.缓慢生长分枝杆菌前体16S rRNA的核苷酸序列和二级结构
Microbiology (Reading). 1994 Jan;140 ( Pt 1):123-32. doi: 10.1099/13500872-140-1-123.
8
Mycobacterium interjectum, a new species isolated from a patient with chronic lymphadenitis.插入分枝杆菌，从一名慢性淋巴结炎患者中分离出的新菌种。
J Clin Microbiol. 1993 Dec;31(12):3083-9. doi: 10.1128/jcm.31.12.3083-3089.1993.
9
CLUSTAL: a package for performing multiple sequence alignment on a microcomputer.CLUSTAL：一个用于在微型计算机上进行多序列比对的程序包。
Gene. 1988 Dec 15;73(1):237-44. doi: 10.1016/0378-1119(88)90330-7.
10
Structure of 5S rRNA in actinomycetes and relatives and evolution of eubacteria.放线菌及其亲缘生物中5S核糖体RNA的结构与真细菌的进化
J Mol Evol. 1987;25(3):255-60. doi: 10.1007/BF02100019.
Zotero 插件