Ruan Wei, Lei Yong-Liang, Yao Li-Nong, Zhang Ling-Ling, Liu Xiao-Hong, Xiang Xiao-Qing, Mei Jian-Hua, Zhu Hai-Bo, Yu Yang, Zeng Chang-You
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2014 Feb;32(1):54-7.
To identify the sources of infection and the mode of transmission of a malaria case with unknown origin.
Clinical data of the case were collected and the epidemiological investigation was conducted. The blood samples of the patient and the suspected infection source (blood donor) were detected by microscopy, rapid diagnostic test strip (RDT) and nested PCR.
The patient did not visited malaria endemic areas. After a blood transfusion, the patient had chills and fever, and was confirmed as falciparum malaria by microscopy with bone marrow and peripheral blood smears and RDT. The blood donor was a worker returned from Africa. Before blood donation she was sick like malaria carrier, and took anti-malarial drug. She was then confirmed as falciparum malaria by RDT and microscopy. The blood samples from the patient and the blood donor were diagnosed as falciparum malaria by nested PCR, and the similarity of the small subunit rRNA (SSU rRNA) sequence was 100%, showing they were mix-infected with K1 and MAD20 genotypes of Plasmodium falciparum.
This patient is confirmed P. falciparum infection via blood transfusion from a donor who returned from Africa.
确定一例来源不明疟疾病例的感染源及传播方式。
收集该病例的临床资料并进行流行病学调查。采用显微镜检查、快速诊断试纸条（RDT）和巢式PCR检测患者及疑似感染源（献血者）的血样。
患者未去过疟疾流行区。输血后，患者出现寒战和发热，通过骨髓及外周血涂片显微镜检查和RDT确诊为恶性疟。献血者为一名从非洲返回的务工人员。献血前她有类似疟原虫携带者的症状，并服用了抗疟药物。随后通过RDT和显微镜检查确诊为恶性疟。患者和献血者的血样经巢式PCR诊断为恶性疟，小亚基核糖体RNA（SSU rRNA）序列相似度为100%，表明他们混合感染了恶性疟原虫的K1和MAD20基因型。
该患者经确认是通过输注一名从非洲返回的献血者血液感染了恶性疟原虫。
