Konno-Amano S, Hirao T, Kohno H, Mori H, Sato Y
Shiseido Basic Research Laboratories, Yokohama, Japan.
Int Arch Allergy Appl Immunol. 1989;90(4):338-44. doi: 10.1159/000235050.
The effects of some antiallergic agents on passive air-pouch anaphylaxis (PAPA) in the dorsal skin of rats were investigated by measuring plasma exudation and histamine content in the pouch fluid. Antiallergic agents, disodium cromoglycate (DSCG), tranilast and ketotifen, dose dependently inhibited both plasma exudation and histamine release, except that ketotifen showed a dose-unrelated inhibition of histamine release. An antihistamine, pyrilamine, suppressed plasma exudation without affecting histamine release. A cyclooxygenase inhibitor, indomethacin, and a 5-lipoxygenase inhibitor, nordihydroguaiaretic acid (NDGA), showed no significant effect. A slow-reacting substance of anaphylaxis antagonist, FPL55712, exerted no effect at 0.3 and 3.0 micrograms/ml, but at 50 micrograms/ml it suppressed not only plasma exudation but also histamine release. Dexamethasone suppressed plasma exudation dose dependently without inhibiting histamine release significantly. A beta-stimulant, isoproterenol, and prostaglandin E2 also exerted significant inhibitory effects on plasma exudation but not on histamine release at 1 mg/ml. Theophylline inhibited both plasma exudation and histamine release. These observations indicate that PAPA is useful for studying antiallergic agents.
通过测量大鼠背部皮肤被动气囊过敏反应（PAPA）中的血浆渗出和囊液中的组胺含量，研究了一些抗过敏药物的作用。抗过敏药物色甘酸钠（DSCG）、曲尼司特和酮替芬剂量依赖性地抑制血浆渗出和组胺释放，但酮替芬对组胺释放的抑制与剂量无关。抗组胺药吡苄明抑制血浆渗出而不影响组胺释放。环氧化酶抑制剂吲哚美辛和5-脂氧合酶抑制剂去甲二氢愈创木酸（NDGA）无显著作用。过敏反应慢反应物质拮抗剂FPL55712在0.3和3.0微克/毫升时无作用，但在50微克/毫升时不仅抑制血浆渗出，还抑制组胺释放。地塞米松剂量依赖性地抑制血浆渗出，但对组胺释放无明显抑制作用。β激动剂异丙肾上腺素和前列腺素E2在1毫克/毫升时也对血浆渗出有显著抑制作用，但对组胺释放无作用。茶碱抑制血浆渗出和组胺释放。这些观察结果表明，PAPA对于研究抗过敏药物是有用的。
