子宫内膜异位症女性中CA 125的免疫反应性决定因素。

Mojiminiyi O A, Bramwell M E, Kennedy S H, Shepstone B J, Humm S M, Barlow D H

University Department of Radiology, Radcliffe Infirmary, Oxford.

J Clin Pathol. 1989 Dec;42(12):1272-5. doi: 10.1136/jcp.42.12.1272.

Among 10 patients with endometriosis CA 125 was increased (greater than 35 U/ml) in endometriotic cyst fluid in all the patients, but only two had increased serum concentrations. Gel electrophoresis of serum, endometriotic cyst fluid, and endometriotic tissue resolved the CA 125 immunoreactive fragments from the three sources into bands of similar electrophoretic mobilities. Electrophoresis under reducing and non-reducing conditions showed immunoreactive fragments of apparent masses of 55,000 and 140,000 daltons, respectively. Analysis under reducing conditions did not result in loss of activity. CA 125 antigen is thought to be a high molecular weight glycoprotein complex. As far as is known, this is the first report describing lower molecular weight immunoreactive determinants of CA 125.

在10例子宫内膜异位症患者中，所有患者的子宫内膜异位囊肿液中CA 125均升高（大于35 U/ml），但只有两例血清浓度升高。血清、子宫内膜异位囊肿液和子宫内膜异位组织的凝胶电泳将来自这三个来源的CA 125免疫反应性片段分离成具有相似电泳迁移率的条带。在还原和非还原条件下进行电泳，分别显示出表观质量为55,000和140,000道尔顿的免疫反应性片段。在还原条件下进行分析并未导致活性丧失。CA 125抗原被认为是一种高分子量糖蛋白复合物。据所知，这是第一份描述CA 125低分子量免疫反应性决定簇的报告。