De Buyser M L, Morvan A, Grimont F, el Solh N
Centre National de Référence pour les Staphylocoques, Laboratoire des Staphylocoques et des Streptocoques, Paris, France.
J Gen Microbiol. 1989 Apr;135(4):989-99. doi: 10.1099/00221287-135-4-989.
The rRNA gene restriction pattern sof 110 strains belonging to 12 staphylococcal species have been determined. The strains, isolated from various sources, were epidemiologically unrelated. Total DNA was cleaved with restriction enzymes HindIII and EcoRI, electrophoretically separated and probed with radiolabelled 16S rDNA from Bacillus subtilis inserted in a plasmid vector, pBR322. Fourty-four distinct HindIII patterns and 44 distinct EcoRI patterns were observed. Strains belonging to different species had different patterns. Although distinct patterns were also observed with some species, a core of common bands could be discerned within each species or subspecies. Analysis of the patterns revealed two taxa in Staphylococcus xylosus which were not evident using phenotypic characteristics. Of 18 strains which were difficult to identify using phenotypic schemes, 15 showed patterns typical of known species. The three remaining atypical strains showed unusual patterns and may belong either to a known species, not included in the study, or to a new species. Since various patterns were observed within some species (e.g.S.aureus and S. epidermidis), rRNA gene restriction patterns may have epidemiological, as well as taxonomic interest.
已确定了属于12个葡萄球菌物种的110株菌株的rRNA基因限制性图谱。这些菌株从各种来源分离得到，在流行病学上没有关联。用限制性内切酶HindIII和EcoRI切割总DNA，进行电泳分离，并用插入质粒载体pBR322中的来自枯草芽孢杆菌的放射性标记16S rDNA进行探针杂交。观察到44种不同的HindIII图谱和44种不同的EcoRI图谱。属于不同物种的菌株具有不同的图谱。尽管在一些物种中也观察到了不同的图谱，但在每个物种或亚种中都可以识别出一组共同的条带。对图谱的分析揭示了木糖葡萄球菌中的两个分类单元，这在用表型特征时并不明显。在18株难以用表型分类方案鉴定的菌株中，15株显示出已知物种的典型图谱。其余三株非典型菌株显示出不寻常的图谱，可能属于未包括在本研究中的已知物种，或者属于一个新物种。由于在一些物种（如金黄色葡萄球菌和表皮葡萄球菌）中观察到了各种图谱，rRNA基因限制性图谱可能在流行病学以及分类学方面都具有研究价值。
