De Buyser M L, Morvan A, Aubert S, Dilasser F, el Solh N
Centre National de Référence pour les Staphylocoques, Institut Pasteur, Paris, France.
J Gen Microbiol. 1992 May;138(5):889-99. doi: 10.1099/00221287-138-5-889.
To evaluate a 16S rRNA gene probe for the identification of staphylococcal species and subspecies, we have augmented previous studies involving 12 staphylococcal species by analysing the remaining 16 species currently classified in the genus Staphylococcus. HindIII- and EcoRI-restricted DNA of isolates from validly described species of Staphylococcus was probed with radiolabelled plasmid pBA2 containing 16S rDNA from Bacillus subtilis. The Dice coefficient was used to assess similarity between the 74 HindIII- and the 81 EcoRI-hybridization patterns obtained from a total of 271 isolates belonging to 31 staphylococcal taxa (28 species, of which three include two subspecies). The use of HindIII yielded a better discrimination of the staphylococci than the use of EcoRI. All of the isolates belonging to the same species or subspecies, except S. hyicus isolates, were recovered as homogeneous clusters using their HindIII hybridization patterns. The phenotypically close taxa were clearly distinguished. Thus, the method presented in this study constitutes a powerful tool for the identification of taxa within the genus Staphylococcus.
为评估一种用于鉴定葡萄球菌属物种和亚种的16S rRNA基因探针，我们在之前涉及12种葡萄球菌的研究基础上进行了扩充，分析了目前分类在葡萄球菌属中的其余16个物种。用含有枯草芽孢杆菌16S rDNA的放射性标记质粒pBA2对来自有效描述的葡萄球菌物种的分离株经HindIII和EcoRI酶切的DNA进行杂交检测。采用Dice系数评估从总共271株属于31个葡萄球菌分类单元（28个物种，其中3个物种包含两个亚种）的分离株获得的74种HindIII酶切和81种EcoRI酶切杂交模式之间的相似性。与使用EcoRI相比，使用HindIII对葡萄球菌的鉴别效果更好。除猪葡萄球菌分离株外，所有属于同一物种或亚种的分离株利用其HindIII杂交模式均被归为同质聚类。表型相近的分类单元也能被清楚地区分。因此，本研究中提出的方法是鉴定葡萄球菌属内分类单元的有力工具。
