Zuber Aleksandra, Borowczyk Julia, Zimolag Eliza, Krok Malgorzata, Madeja Zbigniew, Pamula Elzbieta, Drukala Justyna
Laboratory of Cell & Tissue Engineering, Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology and Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, 30-387, Poland.
Cell Mol Biol Lett. 2014 Jun;19(2):297-314. doi: 10.2478/s11658-014-0197-1. Epub 2014 May 13.
Degradable aliphatic polyesters such as polylactides, polyglycolides and their copolymers are used in several biomedical and pharmaceutical applications. We analyzed the influence of poly(L-lactide-co-glycolide) (PLGA) thin films on the adhesion, proliferation, motility and differentiation of primary human skin keratinocytes and fibroblasts in the context of their potential use as cell carriers for skin tissue engineering. We did not observe visible differences in the morphology, focal contact appearance, or actin cytoskeleton organization of skin cells cultured on PLGA films compared to those cultured under control conditions. Moreover, we did not detect biologically significant differences in proliferative activity, migration parameters, level of differentiation, or expression of vinculin when the cells were cultured on PLGA films and tissue culture polystyrene. Our results indicate that PLGA films do not affect the basic functions of primary human skin keratinocytes and fibroblasts and thus show acceptable biocompatibility in vitro, paving the way for their use as biomaterials for skin tissue engineering.
可降解脂肪族聚酯，如聚丙交酯、聚乙交酯及其共聚物，被用于多种生物医学和制药应用中。在聚（L-丙交酯-共-乙交酯）（PLGA）薄膜作为皮肤组织工程细胞载体的潜在用途背景下，我们分析了其对原代人皮肤角质形成细胞和成纤维细胞的黏附、增殖、迁移和分化的影响。与在对照条件下培养的细胞相比，我们未观察到在PLGA薄膜上培养的皮肤细胞在形态、焦点接触外观或肌动蛋白细胞骨架组织方面有明显差异。此外，当细胞在PLGA薄膜和组织培养聚苯乙烯上培养时，我们未检测到增殖活性、迁移参数、分化水平或纽蛋白表达方面具有生物学意义的差异。我们的结果表明，PLGA薄膜不会影响原代人皮肤角质形成细胞和成纤维细胞的基本功能，因此在体外显示出可接受的生物相容性，为其作为皮肤组织工程生物材料的应用铺平了道路。
