Paduszyński Piotr, Aleksander-Konert Ewelina, Zajdel Alicja, Wilczok Adam, Jelonek Katarzyna, Witek Andrzej, Dzierżewicz Zofia
Department of Biopharmacy, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Katowice, Poland.
Centre of Polymer and Carbon Materials, Polish Academy of Sciences, Zabrze, Poland.
Cell Mol Biol Lett. 2016 Aug 24;21:14. doi: 10.1186/s11658-016-0012-2. eCollection 2016.
In cartilage tissue regeneration, it is important to develop biodegradable scaffolds that provide a structural and logistic template for three-dimensional cultures of chondrocytes. In this study, we evaluated changes in expression of cartilaginous genes during in vitro chondrogenic differentiation of WJ-MSCs on PLGA scaffolds.
The biocompatibility of the PLGA material was investigated using WJ-MSCs by direct and indirect contact methods according to the ISO 10993-5 standard. PLGA scaffolds were fabricated by the solvent casting/salt-leaching technique. We analyzed expression of chondrogenic genes of WJ-MSCs after a 21-day culture.
The results showed the biocompatibility of PLGA and confirmed the usefulness of PLGA as material for fabrication of 3D scaffolds that can be applied for WJ-MSC culture. The in vitro penetration and colonization of the scaffolds by WJ-MSCs were assessed by confocal microscopy. The increase in cell number demonstrated that scaffolds made of PLGA copolymers enabled WJ-MSC proliferation. The obtained data showed that as a result of chondrogenesis of WJ-MSCs on the PLGA scaffold the expression of the key markers collagen type II and aggrecan was increased.
The observed changes in transcriptional activity of cartilaginous genes suggest that the PLGA scaffolds may be applied for WJ-MSC differentiation. This primary study suggests that chondrogenic capacity of WJ-MSCs cultured on the PLGA scaffolds can be useful for cell therapy of cartilage.
在软骨组织再生中，开发可生物降解的支架很重要，这种支架可为软骨细胞的三维培养提供结构和逻辑模板。在本研究中，我们评估了WJ间充质干细胞在PLGA支架上进行体外软骨形成分化过程中软骨基因表达的变化。
根据ISO 10993 - 5标准，采用直接和间接接触法，使用WJ间充质干细胞研究PLGA材料的生物相容性。通过溶剂浇铸/盐析技术制备PLGA支架。我们分析了培养21天后WJ间充质干细胞软骨形成基因的表达。
结果显示了PLGA的生物相容性，并证实了PLGA作为可用于WJ间充质干细胞培养的三维支架制造材料的实用性。通过共聚焦显微镜评估了WJ间充质干细胞对支架的体外穿透和定植情况。细胞数量的增加表明由PLGA共聚物制成的支架能够使WJ间充质干细胞增殖。获得的数据表明，由于WJ间充质干细胞在PLGA支架上发生软骨形成，关键标志物II型胶原蛋白和聚集蛋白聚糖的表达增加。
观察到的软骨基因转录活性变化表明，PLGA支架可用于WJ间充质干细胞的分化。这项初步研究表明，在PLGA支架上培养的WJ间充质干细胞的软骨形成能力可用于软骨细胞治疗。
