Wen You-Liang, Li Zhi, Liang Xing-Sen, Yang Bo
Zhongguo Gu Shang. 2014 Feb;27(2):153-6.
To explore the effects of sacral canal injection on nerve root local inflammatory factors in rat model with lumbar disc herniation, in order to identify its mechanism of treatment.
Forty-eight male SD rats were randomly divided into sham operation group(group A), model group (group B), Chinese medicine group(group C) and western medicine group(group D). There were 12 rats in each group. The model of lumbar disc herniation was established using compression and inflammatory stimulation in group B, C, D. All rats were given epidural catheterization and group A and B with physiological saline (1 ml/kg), group C with mixed liquor of 2% lidocaine and compound Danshen injections and physiological saline (2:2: 16) and group D with mixed liquor of 2% lidocaine and triamcinolone acetonide injection and physiological saline (2:2:16), once a week for a total of three treatments. Four rats were killed every 1 week after injection for once, and the inflammatory factors of tumor necrosis factor (TNF-alpha), prostaglandin E2 (PGE2), interleukin-l (IL-1) and interleukin-6 (IL-6) were detected by ELISA method.
The levels of TNF-alpha, PGE2, IL-1 and IL-6 in compressed nerve tissues in group B were increased than those of group A (P < 0.01). The levels of PGE2, IL-1 and IL-6 in group C and D were decreased than those of group B, and group D was much less(P<0.05). There was no significant difference in level of TNF-alpha among group B, C, D (P > 0.05).
Compound compression with inflammatory stimulation can lead to massive release of inflammatory mediators, such as TNF-alpha, PGE2, IL-1 and IL-6. Both injection with compound Danshen injections and triamcinolone acetonide injections by sacral canal can reduce the levels of part inflammatory mediators (PGE2, IL-1 and IL-6), and the effect of Glucocorticoid is better than Danshen (P < 0.05).
探讨骶管注射对腰椎间盘突出症大鼠模型神经根局部炎症因子的影响，以明确其治疗机制。
将48只雄性SD大鼠随机分为假手术组（A组）、模型组（B组）、中药组（C组）和西药组（D组），每组12只。B、C、D组采用压迫和炎性刺激建立腰椎间盘突出症模型。所有大鼠均行硬膜外置管，A组和B组给予生理盐水（1 ml/kg），C组给予2%利多卡因与复方丹参注射液及生理盐水的混合液（2∶2∶16），D组给予2%利多卡因与曲安奈德注射液及生理盐水的混合液（2∶2∶16），每周1次，共治疗3次。注射后每周处死4只大鼠，采用酶联免疫吸附测定（ELISA）法检测肿瘤坏死因子（TNF-α）、前列腺素E2（PGE2）、白细胞介素-1（IL-1）和白细胞介素-6（IL-6）等炎症因子。
B组受压神经组织中TNF-α、PGE2、IL-1和IL-6水平高于A组（P<0.01）。C组和D组PGE2、IL-1和IL-6水平低于B组，且D组降低更明显（P<0.05）。B、C、D组TNF-α水平差异无统计学意义（P>0.05）。
复合压迫并炎性刺激可导致TNF-α、PGE2、IL-1和IL-6等炎性介质大量释放。骶管注射复方丹参注射液和曲安奈德注射液均可降低部分炎性介质（PGE2、IL-1和IL-6）水平，且糖皮质激素的效果优于丹参（P<0.05）。
