Jiang Haoran, Han Shasha, Guo Chuanlong, Liu Ting, Wu Xianggen
State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences , Qingdao, China .
J Ocul Pharmacol Ther. 2014 Aug;30(6):482-8. doi: 10.1089/jop.2013.0178. Epub 2014 May 14.
To evaluate the effectiveness of balofloxacin for treatment of experimental Staphylococcus aureus keratitis.
In vitro testing compared the cellular toxicity of and bacterial susceptibility to balofloxacin and levofloxacin in human corneal epithelial cells (HCECs). For in vivo testing, experimental bacterial keratitis was induced and treated with balofloxacin eye drops (0.5%) and levofloxacin eye drops (0.5%).
In vitro toxicity examinations showed that balofloxacin, as well as levofloxacin, had low cytotoxicity in HCECs. Balofloxacin eye drops (0.5%) also showed a similar relative cytotoxicity to levofloxacin eye drops (0.5%). In bacterial susceptibility examinations, both balofloxacin and levofloxacin significantly reduced S. aureus compared with the untreated control (P<0.001 for both balofloxacin and levofloxacin). Balofloxacin was more effective than levofloxacin in the treatment of S. aureus bacterial keratitis (P<0.05). In experimental bacterial keratitis treatment testing, balofloxacin was also more effective than levofloxacin with respect to the parameters of physiological score, histological observation, and bacterial quantitation (P<0.05).
Balofloxacin was safe in the treatment of S. aureus bacterial keratitis, and more effective than levofloxacin. Therefore, balofloxacin was shown to have potential clinical value in ophthalmic local application.
评估巴洛沙星治疗实验性金黄色葡萄球菌角膜炎的有效性。
体外试验比较了巴洛沙星和左氧氟沙星对人角膜上皮细胞（HCECs）的细胞毒性及细菌敏感性。体内试验中，诱导实验性细菌性角膜炎，并用0.5%的巴洛沙星滴眼液和0.5%的左氧氟沙星滴眼液进行治疗。
体外毒性检查显示，巴洛沙星和左氧氟沙星在HCECs中的细胞毒性均较低。0.5%的巴洛沙星滴眼液与0.5%的左氧氟沙星滴眼液的相对细胞毒性相似。在细菌敏感性检查中，与未治疗的对照组相比，巴洛沙星和左氧氟沙星均能显著减少金黄色葡萄球菌（巴洛沙星和左氧氟沙星的P均<0.001）。在治疗金黄色葡萄球菌性角膜炎方面，巴洛沙星比左氧氟沙星更有效（P<0.05）。在实验性细菌性角膜炎治疗试验中，在生理评分、组织学观察和细菌定量参数方面，巴洛沙星也比左氧氟沙星更有效（P<0.05）。
巴洛沙星治疗金黄色葡萄球菌性角膜炎安全，且比左氧氟沙星更有效。因此，巴洛沙星在眼科局部应用中显示出潜在的临床价值。
