Lee C, Keefer M, Zhao Z W, Kroes R, Berg L, Liu X X, Sensibar J
Department of Urology, Northwestern University Medical School, Chicago, Illinois 60611.
J Androl. 1989 Nov-Dec;10(6):432-8. doi: 10.1002/j.1939-4640.1989.tb00134.x.
Two-dimensional protein profiles of human semen, prostatic fluid, and seminal vesicle fluid were compared to demonstrate changes in the protein composition of human semen before and after liquefaction. Semen specimens were obtained from a volunteer. Prostatic fluid specimens were collected by rectal massage from patients visiting a urology clinic. Samples of seminal vesicle fluid were collected by needle aspiration from isolated seminal vesicles, which were removed at surgery. All specimens were prepared and processed according to the ISO-DALT system for separation of proteins in two-dimensional gels. Following electrophoresis, protein spots in the gels were visualized by silver staining. Prostatic fluid and seminal vesicle fluid showed their characteristic protein profiles. The protein profile of human semen contained specific proteins of both prostatic fluid and seminal vesicle fluid. One major group of proteins in seminal vesicle fluid (Mw 28,000-68,000 daltons), designated as seminal vesicle-specific antigen, was observed in freshly ejaculated human semen, but disappeared from the two-dimensional profile when the ejaculate was allowed to stand at room temperature for 30 min. When prostatic fluid or prostate-specific antigen was mixed with seminal vesicle fluid and incubated at 37C for 30 min, the seminal vesicle-specific antigen also disappeared from the two-dimensional profiles. The findings indicate that seminal vesicle-specific antigen, a group of predominant proteins in seminal vesicle fluid, is the structural component of seminal coagulum, and that prostate-specific antigen is the enzyme which digests seminal vesicle-specific antigen and liquifies semen coagulum.
比较了人类精液、前列腺液和精囊液的二维蛋白质谱，以证明人类精液液化前后蛋白质组成的变化。精液标本取自一名志愿者。前列腺液标本通过直肠按摩从泌尿科门诊患者中采集。精囊液样本通过针吸从分离的精囊中采集，这些精囊在手术中被切除。所有标本均按照ISO-DALT系统制备和处理，用于在二维凝胶中分离蛋白质。电泳后，凝胶中的蛋白质斑点通过银染进行可视化。前列腺液和精囊液显示出它们特有的蛋白质谱。人类精液的蛋白质谱包含前列腺液和精囊液的特定蛋白质。在新鲜射出的人类精液中观察到精囊液中的一组主要蛋白质（分子量28,000 - 68,000道尔顿），被指定为精囊特异性抗原，但当精液在室温下静置30分钟时，该抗原从二维图谱中消失。当前列腺液或前列腺特异性抗原与精囊液混合并在37℃孵育30分钟时，精囊特异性抗原也从二维图谱中消失。这些发现表明，精囊特异性抗原是精囊液中的一组主要蛋白质，是精液凝固物的结构成分，而前列腺特异性抗原是消化精囊特异性抗原并使精液凝固物液化的酶。
