Re M C, Furlini G, La Placa M
Institute of Microbiology, University of Bologna, Italy.
J Virol Methods. 1989 Dec;26(3):313-7. doi: 10.1016/0166-0934(89)90113-4.
A mild heat-shock (10 min at 44 degrees C), performed just before seeding on healthy PBL used for co-cultivation, allows the detection of viral markers (p24 core antigen and reverse transcriptase) in a very short time. This suggests that heat-shocked PBL can be employed routinely for the rapid detection of HIV-1 in clinical samples.
在接种用于共培养的健康外周血淋巴细胞（PBL）之前，进行一次温和的热休克处理（44摄氏度下10分钟），能够在极短时间内检测到病毒标志物（p24核心抗原和逆转录酶）。这表明热休克处理后的PBL可常规用于临床样本中HIV-1的快速检测。
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