Tremblay M, Numazaki K, Goldman H, Wainberg M A
Lady Davis Institute, Sir Mortimer B. Davis-Jewish General Hospital, Montreal, Quebec, Canada.
J Acquir Immune Defic Syndr (1988). 1990;3(4):356-60.
We have succeeded in infecting human thymic lymphocytes with both the HIV-IIIB laboratory strain of HIV-1 as well as with a clinical isolate of this virus. Thymic lymphocytes were at least as susceptible to infection by HIV-1 as were cord blood lymphocytes, but appeared to display somewhat greater resistance to the cytopathic effects of the virus. As measured variously by each of indirect immunofluorescence for detection of viral p17, antigen capture assay for the presence of viral p24 in culture fluids, and levels of viral reverse transcriptase activity in culture fluids, infection of thymic lymphocytes could be detected as early as 2 days after infection by HIV-1, and persisted through at least 14 days of tissue culture maintenance. These findings suggest that thymic lymphocytes may be susceptible to infection by HIV-1 in vivo, and may also be relevant to our understanding of HIV-1-induced pathogenesis, particularly in pediatric populations.
我们已成功地用HIV-1的HIV-IIIB实验室毒株以及该病毒的一种临床分离株感染了人类胸腺淋巴细胞。胸腺淋巴细胞对HIV-1感染的易感性至少与脐血淋巴细胞相同，但似乎对该病毒的细胞病变效应表现出稍强的抵抗力。通过检测病毒p17的间接免疫荧光、检测培养液中病毒p24的抗原捕获试验以及培养液中病毒逆转录酶活性水平等多种方法测量，早在被HIV-1感染后2天就能检测到胸腺淋巴细胞的感染，并且在组织培养维持至少14天的时间里持续存在。这些发现表明，胸腺淋巴细胞在体内可能易受HIV-1感染，这也可能与我们对HIV-1诱导的发病机制的理解相关，特别是在儿童群体中。
