AlFaisal Abdul Hussein Moyet, Al-Ramahi Intesar Jawad Kahdoom, Abdul-Hassan Ismail Abdul Redah
Genetic Engineering and Biotechnology Institute, Baghdad, Iraq.
Al-Razi Centre for Medical Diagnostic kits Production, Ministry of Industry, Baghdad, Iraq.
Comp Clin Path. 2012 Dec 28;23(3):683-688. doi: 10.1007/s00580-012-1671-7. eCollection 2014.
Micronucleus (MN) assay has been extensively used in detection of DNA damage, instability in cancer, and genetic disorders. In the current study, MN, binucleated cells, and nuclear division index (NDI) were investigated in Iraqi patients with thyroid disorders. The results indicated significantly ( < 0.05) increased binucleated cells with micronucleus (BNMN) frequencies in thyroid cancer group (37.58 ± 3.07) versus other thyroid disorder groups (6.60 ± 1.29, 14.90 ± 1.69, 15.56 ± 1.76). On the other hand, the frequency of micronucleus per 1,000 and the NDI were significantly ( < 0.05) decreased in hypothyroidism (MN 1.55 ± 0.36) (NDI 0.009 ± 0.001) versus other thyroid disorder groups (MN: 6.05 ± 0.97, 6.09 ± 0.53, 5.34 ± 0.56) (NDI: 0.049 ± 0.003, 0.032 ± 0.002, 0.025 ± 0.002), with no difference versus healthy group (0.0 ± 0.0). The number of BNMN and MN are parallel to the severity of thyroid disorders which were 6.60 ± 1.29, 14.90 ± 1.69, 15.56 ± 1.76, and 37.58 ± 3.07 for hypothyroidism, thyroid toxic goiter, thyroid nontoxic goiter, and thyroid cancer, respectively. The number of BNMN and MN are parallel to the severity of thyroid disorders which were 6.60 ± 1.29, 14.90 ± 1.69, 15.56 ± 1.76, and 37.58 ± 3.07 for hypothyroidism, thyroid toxic goiter, thyroid nontoxic goiter, and thyroid cancer, respectively. The results also indicate that there were no significant differences among age and sex groups as related with BNMN formation within each thyroid disorder groups and healthy control group.
微核（MN）检测已广泛应用于DNA损伤、癌症中的不稳定性以及遗传疾病的检测。在本研究中，对伊拉克甲状腺疾病患者的微核、双核细胞和核分裂指数（NDI）进行了调查。结果表明，甲状腺癌组中双核细胞伴微核（BNMN）频率显著（<0.05）升高（37.58±3.07），而其他甲状腺疾病组分别为（6.60±1.29、14.90±1.69、15.56±1.76）。另一方面，甲状腺功能减退症患者每1000个细胞中的微核频率和NDI显著（<0.05）降低（MN 1.55±0.36）（NDI 0.009±0.001），与其他甲状腺疾病组相比（MN：6.05±0.97、6.09±0.53、5.34±0.56）（NDI：0.049±0.003、0.032±0.002、0.025±0.002），与健康组相比无差异（0.0±0.0）。BNMN和MN的数量与甲状腺疾病的严重程度呈平行关系，甲状腺功能减退症、甲状腺毒性甲状腺肿、甲状腺非毒性甲状腺肿和甲状腺癌的BNMN和MN数量分别为6.60±1.29、14.90±1.69、15.56±1.76和37.58±3.07。结果还表明，在每个甲状腺疾病组和健康对照组中，年龄和性别组之间与BNMN形成相关的差异不显著。
