Zhang Guangbin, Tang Yuhai, Shang Jian, Wang Zhongcheng, Yu Hua, Du Wei, Fu Qiang
School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, People's Republic of China; Institute of Analytical Science, Xi'an Jiaotong University, Xi'an, 710061, Shaanxi, People's Republic of China.
Luminescence. 2015 Feb;30(1):102-9. doi: 10.1002/bio.2698. Epub 2014 May 16.
A new method for the detection of β2 adrenergic agonists was developed based on the chemiluminescence (CL) reaction of β2 adrenergic agonist with potassium ferricyanide-luminol CL. The effect of β2 adrenergic agonists including isoprenaline hydrochloride, salbutamol sulfate, terbutaline sulfate and ractopamine on the CL intensity of potassium ferricyanide-luminol was discovered. Detection of the β2 adrenergic agonist was carried out in a flow system. Using uniform design experimentation, the influence factors of CL were optimized. The optimal experimental conditions were 1 mmol/L of potassium ferricyanide, 10 µmol/L of luminol, 1.2 mmol/L of sodium hydroxide, a flow speed of 2.6 mL/min and a distance of 1.2 cm from 'Y2 ' to the flow cell. The linear ranges and limit of detection were 10-100 and 5 ng/mL for isoprenaline hydrochloride, 20-100 and 5 ng/mL for salbutamol sulfate, 8-200 and 1 ng/mL for terbutaline sulfate, 20-100 and 4 ng/mL for ractopamine, respectively. The proposed method allowed 200 injections/h with excellent repeatability and precision. It was successfully applied to the determination of three β2 adrenergic agonists in commercial pharmaceutical formulations with recoveries in the range of 96.8-98.5%. The possible CL reaction mechanism of potassium ferricyanide-luminol-β2 adrenergic agonist was discussed from the UV/vis spectra.
基于β2肾上腺素能激动剂与铁氰化钾-鲁米诺化学发光（CL）反应，开发了一种检测β2肾上腺素能激动剂的新方法。发现了包括盐酸异丙肾上腺素、硫酸沙丁胺醇、硫酸特布他林和莱克多巴胺在内的β2肾上腺素能激动剂对铁氰化钾-鲁米诺CL强度的影响。在流动系统中进行β2肾上腺素能激动剂的检测。采用均匀设计实验对CL的影响因素进行了优化。最佳实验条件为：铁氰化钾1 mmol/L、鲁米诺10 μmol/L、氢氧化钠1.2 mmol/L、流速2.6 mL/min以及从“Y2”到流通池的距离为1.2 cm。盐酸异丙肾上腺素的线性范围和检测限分别为10 - 100 ng/mL和5 ng/mL，硫酸沙丁胺醇为20 - 100 ng/mL和5 ng/mL，硫酸特布他林为8 - 200 ng/mL和1 ng/mL，莱克多巴胺为20 - 100 ng/mL和4 ng/mL。所提出的方法允许每小时进样200次，具有出色的重复性和精密度。该方法成功应用于市售药物制剂中三种β2肾上腺素能激动剂的测定，回收率在96.8% - 98.5%范围内。从紫外/可见光谱讨论了铁氰化钾-鲁米诺-β2肾上腺素能激动剂可能的CL反应机理。
