Chen Xu, Luo Yong, Shi Bo, Gao Zhigang, Du Yuguang, Liu Xianming, Zhao Weijie, Lin Bingcheng
Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China; School of Pharmaceutical Science and Technology and State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, China.
Electrophoresis. 2015 Apr;36(7-8):986-93. doi: 10.1002/elps.201400412. Epub 2015 Mar 11.
β-Agonists are a group of illegal feed additives. In this paper, it was found that the light emission produced by the oxidation of luminol by potassium ferricyanide was enhanced by the β-agonists (ractopamine, salbutamol, and terbutaline). Based on chemiluminescence phenomenon, a novel, rapid, and sensitive microflow injection analysis system on a microfluidic glass chip was established for determination of the β-agonists. The chip was fabricated from two glass plates (64 mm × 32 mm) with microchannels of 200 μm width and 100 μm depth. The detection limits were achieved at 2.0 × 10(-8) mol/L of ractopamine, 1.0 × 10(-8) mol/L of terbutaline and 5.0 × 10(-7) mol/L of salbutamol. In this report, our method was applied for determination of the β-agonists in swine hair from three different sources with satisfactory results.
β-激动剂是一类非法饲料添加剂。本文发现，β-激动剂（莱克多巴胺、沙丁胺醇和特布他林）可增强铁氰化钾氧化鲁米诺产生的发光。基于化学发光现象，建立了一种新型、快速、灵敏的微流控玻璃芯片微流控注射分析系统用于测定β-激动剂。该芯片由两块玻璃片（64 mm×32 mm）制成，微通道宽度为200μm，深度为100μm。莱克多巴胺的检测限为2.0×10(-8) mol/L，特布他林的检测限为1.0×10(-8) mol/L，沙丁胺醇的检测限为5.0×10(-7) mol/L。在本报告中，我们的方法用于测定来自三种不同来源猪毛中的β-激动剂，结果令人满意。
