Li Qi, Reed David A, Min Liu, Gopinathan Gokul, Li Steve, Dangaria Smit J, Li Leo, Geng Yajun, Galang Maria-Therese, Gajendrareddy Praveen, Zhou Yanmin, Luan Xianghong, Diekwisch Thomas G H
Department of Implantology, Stomatological Hospital, Jilin University, Changchun 130021, Jilin, China.
UIC Brodie Laboratory for Craniofacial Genetics, Chicago, IL 60612, USA.
Int J Mol Sci. 2014 May 14;15(5):8509-25. doi: 10.3390/ijms15058509.
Freeze-drying is an effective means to control scaffold pore size and preserve its composition. The purpose of the present study was to determine the applicability of lyophilized Platelet-rich fibrin (LPRF) as a scaffold for craniofacial tissue regeneration and to compare its biological effects with commonly used fresh Platelet-rich fibrin (PRF). LPRF caused a 4.8-fold±0.4-fold elevation in Runt-related transcription factor 2 (Runx2) expression in alveolar bone cells, compared to a 3.6-fold±0.2-fold increase when using fresh PRF, and a more than 10-fold rise of alkaline phosphatase levels and mineralization markers. LPRF-induced Runx2 expression only occurred in alveolar bone and not in periodontal or dental follicle cells. LPRF also caused a 1.6-fold increase in osteoblast proliferation (p<0.001) when compared to fresh PRF. When applied in a rat craniofacial defect model for six weeks, LPRF resulted in 97% bony coverage of the defect, compared to 84% for fresh PRF, 64% for fibrin, and 16% without scaffold. Moreover, LPRF thickened the trabecular diameter by 25% when compared to fresh PRF and fibrin, and only LPRF and fresh PRF resulted in the formation of interconnected trabeculae across the defect. Together, these studies support the application of lyophilized PRF as a biomimetic scaffold for craniofacial bone regeneration and mineralized tissue engineering.
冷冻干燥是控制支架孔径和保持其成分的有效方法。本研究的目的是确定冻干富血小板纤维蛋白（LPRF）作为颅面组织再生支架的适用性，并将其生物学效应与常用的新鲜富血小板纤维蛋白（PRF）进行比较。与使用新鲜PRF时3.6倍±0.2倍的增加相比，LPRF使牙槽骨细胞中与Runt相关的转录因子2（Runx2）的表达升高了4.8倍±0.4倍，碱性磷酸酶水平和矿化标记物增加了10倍以上。LPRF诱导的Runx2表达仅发生在牙槽骨中，而不在牙周或牙囊细胞中。与新鲜PRF相比，LPRF还使成骨细胞增殖增加了1.6倍（p<0.001）。当应用于大鼠颅面缺损模型六周时，LPRF导致缺损的骨覆盖率达到97%，而新鲜PRF为84%，纤维蛋白为64%，无支架时为16%。此外，与新鲜PRF和纤维蛋白相比，LPRF使小梁直径增加了25%，只有LPRF和新鲜PRF导致缺损处形成相互连接的小梁。总之，这些研究支持将冻干PRF作为颅面骨再生和矿化组织工程的仿生支架应用。
