Lee Jung Seung, Lee Kihong, Moon Sung-Hwan, Chung Hyung-Min, Lee Jun Hyup, Um Soong Ho, Kim Dong-Ik, Cho Seung-Woo
Department of Biotechnology, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-749, Republic of Korea.
Macromol Biosci. 2014 Aug;14(8):1181-9. doi: 10.1002/mabi.201400052. Epub 2014 May 16.
Enhanced endothelialization of tissue-engineered blood vessels is essential for vascular regeneration and function of engineered vessels. In this study, mussel-inspired surface chemistry of polydopamine (pDA) coatings are applied to functionalize decellularized vein matrix (DVM) with extracellular matrix-derived cell adhesion peptides (RGD and YIGSR). DVMs engineered with pDA-peptides enhance focal adhesion, metabolic activity, and endothelial differentiation of human endothelial progenitor cells (EPCs) derived from cord blood and embryonic stem cells compared with EPCs on non-coated or pDA-coated DVMs. These results indicate that pDA-peptide functionalization may contribute to enhanced, rapid endothelialization of DVM surfaces by promoting adhesion, proliferation, and differentiation of circulating EPCs. Ultimately, this approach may be useful for improving in vivo patency and function of decellularized matrix-based blood vessels.
组织工程血管的内皮化增强对于血管再生和工程血管的功能至关重要。在本研究中，受贻贝启发的聚多巴胺（pDA）涂层表面化学被用于用细胞外基质衍生的细胞粘附肽（RGD和YIGSR）对脱细胞静脉基质（DVM）进行功能化。与未涂层或pDA涂层的DVM上的内皮祖细胞（EPC）相比，用pDA-肽工程化的DVM增强了源自脐带血和胚胎干细胞的人内皮祖细胞（EPC）的粘着斑、代谢活性和内皮分化。这些结果表明，pDA-肽功能化可能通过促进循环EPC的粘附、增殖和分化，有助于增强DVM表面的快速内皮化。最终，这种方法可能有助于改善基于脱细胞基质的血管在体内的通畅性和功能。
